FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001322041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485421
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT142562.9368321518846527No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT65281.3448120291458343TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG47310.9746179089903403TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT35230.7257617614400695RNA PCR Primer, Index 3 (95% over 23bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA18200.37493227528269274RNA PCR Primer, Index 3 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.3174563935223239No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT14990.3088041102465695TruSeq Adapter, Index 3 (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.285319341355236No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC9280.19117425904524116No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG8940.18417002972677324TruSeq Adapter, Index 3 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8400.17304566551508896No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT7270.14976690336841628No Hit
ATACAATATAGCATCAATGCTGAATAGCATGATTATGTGCAATACATAAA5450.11227367584014701No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC4870.10032528464981944Illumina PCR Primer Index 12 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)