FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69650
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT1041714.956209619526204No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT52417.524766690595837TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG39175.623833452979182TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT30824.424982053122757RNA PCR Primer, Index 3 (95% over 23bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA16012.2986360373295045RNA PCR Primer, Index 3 (96% over 28bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT12431.7846374730796841TruSeq Adapter, Index 3 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9381.3467336683417086No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG8691.2476669059583632TruSeq Adapter, Index 3 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8051.1557788944723617No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC6660.9562096195262024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.7681263460157932No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT5080.7293610911701364No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.6489590811198851No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC4090.587221823402728Illumina PCR Primer Index 12 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG2810.40344580043072503No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC2580.3704235463029433No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.3173007896625987No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.22828427853553482No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.208183776022972No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT1380.1981335247666906TruSeq Adapter, Index 3 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT1300.18664752333094042No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.18664752333094042Clontech SMART CDS Primer II A (100% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.18521177315147164No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.1722900215362527No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGTAGAGG1190.1708542713567839No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1180.16941852117731515No Hit
GTACATGGGTGAGGCTGGATTTCCTTTGTTTAAGTCCTAGTTTCCCATTT940.1349605168700646No Hit
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC920.13208901651112706No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA910.13065326633165827No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA870.1249102656137832No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG850.12203876525484565No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA790.11342426417803302TruSeq Adapter, Index 3 (96% over 28bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT760.10911701363962671Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.1048097631012204No Hit
GTTATATAATTTACCGTAGGACACTGAGCTGATAAATGGGAAACTAGGAC730.1048097631012204No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.10337401292175162No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT720.10337401292175162No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG710.10193826274228285No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)