FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001322085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684114
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT22310.32611523810359094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22190.3243611444876146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.24177257006873124No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT14390.21034505944915613RNA PCR Primer, Index 17 (95% over 21bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG11660.17043942968569567RNA PCR Primer, Index 17 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.14632064246602175No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT8810.1287797063062589RNA PCR Primer, Index 17 (95% over 23bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG8040.11752427227041107No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA6910.10100655738663439No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)