FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001322107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589297
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154700.9733863462902151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112400.7072309329219145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76760.4829808399562825No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.12754066735166553Clontech SMART CDS Primer II A (100% over 21bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG18950.11923510835293843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.10029591699978041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)