FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001322131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401248
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51691.2882307201531222Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35290.8795059414626365No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28480.7097854693356727Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26820.6684145466145626No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT24070.599878379456097No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21750.5420587766169551Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18870.47028271792008935No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16050.40000199377940826Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14990.37358441662014513Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14030.34965906372118993Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13020.3244875986920807Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12760.3180078156152803Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA12120.3020575803493102Clontech Universal Primer Mix Long (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT11510.28685501236143235TruSeq Adapter, Index 1 (95% over 21bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG10170.2534592072733073Clontech Universal Primer Mix Long (96% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10100.2517146502910918No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9840.24523486721429144Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA9330.23252452348672142Clontech Universal Primer Mix Long (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9090.22654318526198264No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC8490.21158983970013556Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC7460.18591992981896482Clontech Universal Primer Mix Long (96% over 25bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT7420.18492304011484167RNA PCR Primer, Index 1 (95% over 23bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA6460.16099768721588645Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC5780.14405056224579313Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT5770.14380133981976234Clontech Universal Primer Mix Long (96% over 25bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG5760.14355211739373155TruSeq Adapter, Index 1 (96% over 28bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG5560.1385676688731159Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC5400.13458011005662332Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG5150.12834954940585372Clontech Universal Primer Mix Long (96% over 25bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4780.11912831964271474No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT4750.11838065236462238Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG4180.1041749740808677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4130.10292886195071378No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)