FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001322169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406536
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT37170.914310171792904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.6543085975165791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22710.5586221146466733No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT19070.46908514867072043RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG18400.4526044433949269RNA PCR Primer, Index 23 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.3123954582128028No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT11830.290995139421847RNA PCR Primer, Index 23 (96% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9930.24425881102780567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9530.23441958399748114No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA7250.1783359899246315RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT4860.11954660841844264RNA PCR Primer, Index 23 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4310.10601767125174646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)