FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148537
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT41172.771699980476245No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT20611.3875330725677777RNA PCR Primer, Index 31 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13610.9162700202643111No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13580.914250321468725No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT13110.8826083736712065RNA PCR Primer, Index 31 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.8368285343045839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.6483233133831975No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG9580.644957148723887RNA PCR Primer, Index 31 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.45443222900691416No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT4720.3177659438389088RNA PCR Primer, Index 31 (96% over 27bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG3290.22149363458262925RNA PCR Primer, Index 38 (95% over 24bp)
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.20196987955862852No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA2260.15215064260083347RNA PCR Primer, Index 31 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC2170.1460915462140746No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2090.14070568275917783Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT1900.12791425705379805RNA PCR Primer, Index 31 (95% over 21bp)
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC1800.1211819277351771RNA PCR Primer, Index 38 (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.1191622289395908No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.11377636548469405No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1580.10637080323421101Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.10435110443862473Clontech SMART CDS Primer II A (100% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)