FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001322207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740764
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133020.7641472365007549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97500.5600988991040715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59980.34456135352063805No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT49850.2863685140547484No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT27010.15516175656206124RNA PCR Primer, Index 33 (95% over 22bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG25980.1492448143458849No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG24400.14016833987835225No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG22480.1291387000190721RNA PCR Primer, Index 33 (96% over 29bp)
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG19300.1108708590021393No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT18690.10736665050518049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)