FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001322218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756415
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92080.5242496790337136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62830.3577172820774134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39460.22466216697078992No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22490.12804490965973303Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG22480.12798797550692748No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA20470.11654421079300735No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19760.11250188594381168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)