FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001322219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420119
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62380.4392589635093961No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT50550.3559560853703105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48350.34046442586853637No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC29030.2044194887893198No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG28910.2035744891801321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.1979411584522142No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA27160.19125157821281175No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT25970.1828719987550339TruSeq Adapter, Index 3 (95% over 21bp)
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT25820.18181574924354932No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG25310.17822450090450168TruSeq Adapter, Index 3 (96% over 28bp)
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA22260.15674742750431478No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA22170.15611367779742402No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA20640.1453399327802811No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG20400.14364993356190572No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT19380.13646743688381044No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG19380.13646743688381044No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT19350.13625618698151354No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC19270.13569285390872174No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA18210.12822869069423057No Hit
TCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTTT17800.12534160869617264No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA17690.12456702572108394No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA17630.1241445259164901No Hit
TTCTAGGATAGTCAGTAGAATTAGAATTGTGAAGATGATAAGTGTGGAGG17450.12287702650270857No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT16450.11583536309281123RNA PCR Primer, Index 3 (95% over 23bp)
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA16420.11562411319051431No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA16130.1135820308016441No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG16020.11280744782655538No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15850.11161036504687284Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC15790.111187865242279No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC15410.10851203314651801No Hit
CTTCTAGCAAGCCTCGCTAACCTCGCCTTACCCCCCACTATTAACCTACT15180.10689245056224161No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA15080.10618828422125189No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT14610.10287870241860013No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG14560.10252661924810527No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT14410.10147036973662067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)