FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT342.026221692491061Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT221.3110846245530394Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT191.132300357568534Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT171.0131108462455305Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC160.9535160905840285Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT130.7747318235995232Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC110.6555423122765197Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC90.5363528009535161Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC90.5363528009535161Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC90.5363528009535161Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA70.4171632896305125Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT70.4171632896305125Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA70.4171632896305125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA60.3575685339690107Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC60.3575685339690107Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG50.29797377830750893Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA50.29797377830750893Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC50.29797377830750893Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT40.23837902264600713Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.17878426698450536Clontech SMART CDS Primer II A (100% over 21bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30.17878426698450536Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC30.17878426698450536Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20.11918951132300357Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT20.11918951132300357Clontech SMART CDS Primer II A (96% over 26bp)
TCTCGCCGTCGCTTCTTCCCGCGTGGAACCGCACGATGACCGTGTCGAGC20.11918951132300357No Hit
CCCAGTGTGCTGCTTCAAAGTTTATCGAAGCCTCGCCTGGAGTGGTATCA20.11918951132300357No Hit
GTCCAGCAGAGCGCCGCGCTCGCCGTCGACATCGCGCCCTACGCCCCGAC20.11918951132300357No Hit
ACCACAACCCGCCGCCGGCCCTGGAGGTGCAGCGCCCGGAACCGCCGGCG20.11918951132300357No Hit
TATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20.11918951132300357Clontech Universal Primer Mix Long (96% over 28bp)
GTACACTTCCATATACTACAAAAAGAGCGTTTCAAACCTGCTCTAGGAAA20.11918951132300357No Hit
GAACGGAAAGGTGCCGAAGCGCCCTTCCGCACGCGCCTGCATGACATAGG20.11918951132300357No Hit
GGGCAACTCCAGACCCTCCAGGCAGAGAGGACCCAGAAGGAGGAGAGAGG20.11918951132300357No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC20.11918951132300357Clontech Universal Primer Mix Long (96% over 25bp)
GCCGCGTACCACGCTCGTCAAGGCGCCCAGCAGCCAGTAGGCGAAGGCAC20.11918951132300357No Hit
GAACATCCAGAGTATCTGCAAGGTCCGAGGACGCGTGGAGTTCGTGGCGC20.11918951132300357No Hit
CCTTTGCTATTCAGACACAGTCGTGTCCGATGGCACGCAGAGACAAATTA20.11918951132300357No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG20.11918951132300357Clontech Universal Primer Mix Long (96% over 25bp)
GGATACAAGTAATAAAGGAAAATAAAATGTGGTAAGGTGCTTGCTCAGAT20.11918951132300357No Hit
GTTCAATACTCCGGTCACAATATCCTCGAAGGATAAACTAGAGTGTACGG20.11918951132300357No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG20.11918951132300357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTGAACACCTGGCAGCAGTGCCAGGCGTCCGGGGACGACGGCGAACAAA20.11918951132300357No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT20.11918951132300357Clontech SMART CDS Primer II A (96% over 26bp)
GTGAAAAAGGCTGTTATCTTTTCTTTGCTTATGAAGCTTAGTTTCACTGG20.11918951132300357No Hit
CCCGTACGTCGACTTCGGCTTGGCCATCAAGTTGTTCTTCAAGAACTATG20.11918951132300357No Hit
GTGCTGACTTGTGCATTTGCAAATCTTTAGCTAGACACAGAGCGCTGTTT20.11918951132300357No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20.11918951132300357Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC20.11918951132300357Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG20.11918951132300357Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGCTC20.11918951132300357Clontech Universal Primer Mix Long (96% over 27bp)
GCGATAACACCAGCCAGACCATCGCTCTGGAAGGCTTCGCTGATCAGGCT20.11918951132300357No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT20.11918951132300357Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA20.11918951132300357Clontech SMART CDS Primer II A (96% over 26bp)
ATGCTATGGAGCAAGAGAAGCCGGGAGCAAATGACAGAGAGTGGGTGAGG20.11918951132300357No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA20.11918951132300357Clontech Universal Primer Mix Long (96% over 25bp)
GACGTGCTACCGATCGATTCGCGCTTCGACAAGGCCAATTCGTGGCACAC20.11918951132300357No Hit
GAATTGCCATGCGTCGAAAGTCTGCCCTCGGATTTGTAGCTTTGTCCCTG20.11918951132300357No Hit
GATAGACGCCCGGTACATCGACGAAGTCCTTCAGCGTCGCGCGGCCGTCG20.11918951132300357No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC20.11918951132300357Clontech SMART CDS Primer II A (96% over 26bp)
GTATGACAACAGCAGCACTGCCGCAGCCATGACGATCAAACTCGACACCG20.11918951132300357No Hit
GACGTGTAGTCCTCTCAAGGTGAGGGCGTGCAACCCTCTGTTGGCGAGGA20.11918951132300357No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT20.11918951132300357Clontech Universal Primer Mix Long (96% over 25bp)
CCCAAATACTCTCGTCTTGAGTATATTACTGATCATTTCTTTGCTACATA20.11918951132300357No Hit
ACGCCGCGCAGGGAATCAACGACGACATCCTCGACAGCGTTGATGAGTTT20.11918951132300357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11918951132300357No Hit
CCTCCTCGACATCGAACTCGATAATCAGGCTGCTCTTCATGCTTGTCGCC20.11918951132300357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)