FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT583.1659388646288207Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT321.7467248908296942Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT221.2008733624454149Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG201.0917030567685588RNA PCR Primer, Index 31 (96% over 29bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC150.8187772925764192Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT140.7641921397379913Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC140.7641921397379913Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT130.7096069868995634Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC120.6550218340611353Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC120.6550218340611353Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA100.5458515283842794Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG90.49126637554585156Clontech Universal Primer Mix Long (96% over 25bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA80.43668122270742354RNA PCR Primer, Index 31 (96% over 29bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA80.43668122270742354Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT80.43668122270742354Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC80.43668122270742354Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA80.43668122270742354Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC70.38209606986899564Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA60.32751091703056767Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA60.32751091703056767Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT60.32751091703056767Clontech Universal Primer Mix Long (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT60.32751091703056767No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC50.2729257641921397Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA50.2729257641921397Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC50.2729257641921397Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG50.2729257641921397Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG40.21834061135371177Clontech Universal Primer Mix Long (96% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG30.16375545851528384Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA30.16375545851528384Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.16375545851528384Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG30.16375545851528384Clontech Universal Primer Mix Long (96% over 25bp)
GAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAA30.16375545851528384RNA PCR Primer, Index 17 (96% over 27bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30.16375545851528384Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT30.16375545851528384Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG30.16375545851528384Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC30.16375545851528384Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16375545851528384No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG20.10917030567685589Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATAAAAGTGAAATAACTTACCCAATTTCAAATATCAAAGTTACGAATCA20.10917030567685589No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG20.10917030567685589Clontech SMART CDS Primer II A (96% over 26bp)
TGGCCGGGCTCCTCGGAGCGCGCGGCGATGCGCGCCAGCGCGGCGGACTC20.10917030567685589No Hit
ACCTACCTGTTCCTTTAAATTCACAGAGCTATAGAGCTAGAAGGTATCTC20.10917030567685589No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.10917030567685589Clontech SMART CDS Primer II A (96% over 26bp)
ATGCTAGGACGCGCCCCGGAGCTGGGCAACCGCAGCGGCCTGTCTCTTAT20.10917030567685589No Hit
ACGGCAAGTACCGCTTCGACATCGGCTCGGCCGGCGGCCCGACGCAGGCT20.10917030567685589No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA20.10917030567685589Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGGGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT20.10917030567685589Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG20.10917030567685589Clontech SMART CDS Primer II A (96% over 26bp)
GTCCTCGACCGACTCCTTCTGCAAGGAGAGCTTTTGCAGCAGGTTCATCG20.10917030567685589No Hit
CAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGT20.10917030567685589Clontech Universal Primer Mix Long (96% over 28bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
GCCAAATGACGGTCAGTTGGATACCTCTTATCATCCTTCAGTGGTATTAC20.10917030567685589No Hit
CCGACAAGCTCAACGTCGACACGGTGGTGATCCTCGGCGCGCTGCTGGCC20.10917030567685589No Hit
CATATATTGAGACTGATAAGATAAGAGAACTTTCTAAAGATGTTCAGGCT20.10917030567685589No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
GGCCAGTGGCGCCTTTGCCCAAGTTTTGCTCAGGCCCGCTGGGCTGGTTC20.10917030567685589No Hit
GAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAAAAA20.10917030567685589Illumina PCR Primer Index 1 (96% over 27bp)
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA20.10917030567685589TruSeq Adapter, Index 3 (96% over 28bp)
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT20.10917030567685589Clontech SMART CDS Primer II A (96% over 26bp)
AATCGACATTGACAGGCATGATCGACAACGCCAACACCACATTCACCACC20.10917030567685589No Hit
ATCTCCGACAGGACATCATAGTCATCGGCTCCGAACGAAGCTTGACCGTG20.10917030567685589No Hit
ACAGTCGCCTCCTGTGACGGCATCAAGGAGGCGTACCCGATTCTCGGCAC20.10917030567685589No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
GTGTAGAAGGTGTGGTCGAGGTCCACCCGCTTGATCAGCTTGCCGGTGTC20.10917030567685589No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC20.10917030567685589Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10917030567685589No Hit
GCCAGAGACTGCCGGACGGGATACGGGCAACCCGGGGGCGAGTACGAGGC20.10917030567685589No Hit
TATCTACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG20.10917030567685589Clontech SMART CDS Primer II A (96% over 26bp)
GAGCTCAATAACATCATTTCCGCAATTGGTAGCGACCAGTCGGCACCAAC20.10917030567685589No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
GTCTAATCATGGGTCTTTCCAAATTATTTGTTGGCTTGTGTTTAAAATAT20.10917030567685589No Hit
AGAAATTCCTTGAACCCCTTGAGCATTCGACGTCCTTTCTGGGCTGGACA20.10917030567685589No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAAGAGTACAAGCAGTGGTATCAACGCAAGAGTACAAGCAGTGGTAT20.10917030567685589No Hit
CAGTTTGAGTTTAGAATAGTCAGACCGCAGTTGTGCCTCTGTAATTCCAA20.10917030567685589No Hit
AGCGCTTGTTCTCGGGGCCGCTGCGGCCGGGAACGTCGGCGTCGTGCACC20.10917030567685589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
CTTATTTATTTTTTCTTTCTGAGGTATTAAAATATCTGGACTGAGTTTTG20.10917030567685589No Hit
AGGCCCCTCCCGACAACAGGACGATGAGCAGGTCGCGAGGTGTGAGATCT20.10917030567685589No Hit
ACAAGTAACTGACTGGCAGCTTGGCGTTAGGCACGGCTCCTCACGTTCGG20.10917030567685589No Hit
GTTCCGCGTGGGGCTCGCGCTCTATGTCGTCTATTACTACCTCTCGACGG20.10917030567685589No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT20.10917030567685589Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
CTCGAGCACTAGGGCGCGCCATTGACTGCACCGTTCACCGCATTCAGTTC20.10917030567685589No Hit
CGTCGCGACACCCTCTTTGGTGGGCGCCTTCCTTCTCCGAATTCGCGAAC20.10917030567685589No Hit
GAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAA20.10917030567685589Illumina PCR Primer Index 12 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)