FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT432.3471615720524017Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT291.5829694323144103Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT251.3646288209606987Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC160.8733624454148471Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC140.7641921397379913Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT120.6550218340611353Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT120.6550218340611353Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA110.6004366812227074Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC110.6004366812227074Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA110.6004366812227074Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT100.5458515283842794Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA100.5458515283842794Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG90.49126637554585156Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC80.43668122270742354Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA70.38209606986899564Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC70.38209606986899564Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.32751091703056767No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT50.2729257641921397Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC40.21834061135371177Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG40.21834061135371177Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA40.21834061135371177Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG40.21834061135371177Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC30.16375545851528384Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG30.16375545851528384Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT30.16375545851528384Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30.16375545851528384Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16375545851528384No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA30.16375545851528384Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16375545851528384No Hit
TACGACCACTGGGCCATCGAGTACGCCTACGCCCCTCTCGACGGCTTGAG20.10917030567685589No Hit
CTAAACCACCTTTGTTAAGAAAATTCTCAACTTGAAAACGAATATTTCTA20.10917030567685589No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA20.10917030567685589Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGCGTGGGCCTGCGCCTGAACGCCATGGTGCACGACGCCGACGTTCCCGG20.10917030567685589No Hit
CCGCTGGCAAAGCGCTGCTGCTCCGGTTTCGATGCGCCGTACATGCCGTT20.10917030567685589No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
GAACTTAATCAGCCTGCAGTTATTACATTGAGCAATGTGGTTAGGTTTAA20.10917030567685589No Hit
CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20.10917030567685589Clontech SMART CDS Primer II A (96% over 26bp)
CAACAACGCCACCTCAAATGGAGCACGCATTGGAATTACAGAGGCACAAC20.10917030567685589No Hit
GCCGCTGCGGTTGCCCAGCTCCGGGGCGCGTCCTAGCATCTGTCTCTTAT20.10917030567685589No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
ATGGATTGTTACAGTCGATTAAAACAAGCGTATGTTTTTTCATTGACAGG20.10917030567685589No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC20.10917030567685589Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
GTCCTACCCTCAGGAGGCTATTGCTGAGGCGCAGGTAGTCTTTGACGTAG20.10917030567685589No Hit
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGT20.10917030567685589Clontech SMART CDS Primer II A (95% over 24bp)
GCTTATACTCTTGCGTTGATACCACTGCTTGTACTCTTGCGTTGATACCA20.10917030567685589No Hit
ACGTTGGATCTCGTCAATCACGAGACGCCCCTCTTTGGCCGAGCTAAGGA20.10917030567685589No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG20.10917030567685589Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATGTTCGGATTTTCGGATGTTAAATGATTGAAACAGTTTCTATTTAAATG20.10917030567685589No Hit
CGCTTCTCTTGGTCATAGACGCTGACGCCGGTGATGTCTGACGGGAGTAG20.10917030567685589No Hit
CCCTGCACTGAGTACCTGACTGCCGCTTGCCACAGAACCAGCAGCAAACT20.10917030567685589No Hit
GTTTAGTTAATCTGGGGGTGGACGCAGATCAGCGTTTATACCTAGGCCCA20.10917030567685589No Hit
GTCACCGTGTCTTCACCAAGCGAATCACGGTTGTGGAAGCCGGCCACCCG20.10917030567685589No Hit
TTCTATGGCAATGCGCGGTTTGACGACATCACTTTCCAGCTCAGTCCAGA20.10917030567685589No Hit
ACGGCGAGCCGGACACCCTGTCGGTGTCTCCGCAGTTCGAGCAGGCCAAC20.10917030567685589No Hit
GGCTGGACCCCCTGCTCACTTGCTCACACACCCCTCACTGTTCCATGCCT20.10917030567685589No Hit
CAGTCAAAGAGCTTGTTTTGAGTTCCCTGATTCGTAACTTTGATATTTGA20.10917030567685589No Hit
GATTGGGGTTAGCTTCATGGTCATCGTCGTCTTATGACCCTGCAAGTCCA20.10917030567685589No Hit
TTATCCATGTGGTTTCCCTCCAGTCTTGTTCATTATTCAAGTGACTCTCC20.10917030567685589No Hit
CAATAAAGGACATCAAACACAGTCATGAGGCACTAATGACATAAGCAATC20.10917030567685589No Hit
ATACCGAAGCCCGCGACGATGAGCCACTTCTTGCGCGTCAGCCGCGCCGA20.10917030567685589No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG20.10917030567685589Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTGTCGAACGCTGCCATCGCAGATGAACTATGTCTCAGCGAGTCCGCGG20.10917030567685589No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC20.10917030567685589Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAAGATCGACGTCGAGGTGGTGGACATCCTGCGCGACAAGGCCAATCTG20.10917030567685589No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA20.10917030567685589Clontech Universal Primer Mix Long (96% over 25bp)
CAATGATACCGAGGCGGTCTGCGTCACCATCAGTGGCAATGCCGATATCG20.10917030567685589No Hit
ACCAGCGACACCGCCAGGCTGCGCGCCAGGATCGCCGCGACGCGGGTGTC20.10917030567685589No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)