FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT624.065573770491803Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG483.1475409836065573RNA PCR Primer, Index 46 (96% over 28bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT241.5737704918032787Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT201.3114754098360655Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA171.1147540983606559RNA PCR Primer, Index 46 (96% over 28bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA161.0491803278688525Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT161.0491803278688525Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA150.9836065573770493Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC140.9180327868852459Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT130.8524590163934427Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC110.7213114754098361Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC100.6557377049180327Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC100.6557377049180327Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG100.6557377049180327Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG90.5901639344262295Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC90.5901639344262295Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA90.5901639344262295Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC70.45901639344262296Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT60.39344262295081966Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC60.39344262295081966Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT50.32786885245901637Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.32786885245901637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.32786885245901637No Hit
TCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAA40.26229508196721313TruSeq Adapter, Index 4 (96% over 27bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC40.26229508196721313Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA40.26229508196721313Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG40.26229508196721313Clontech Universal Primer Mix Long (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.26229508196721313No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA40.26229508196721313Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG40.26229508196721313Clontech Universal Primer Mix Long (96% over 25bp)
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG40.26229508196721313Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA40.26229508196721313Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30.19672131147540983Clontech Universal Primer Mix Long (96% over 25bp)
ACCTAGTTCATTATAAACCTTTGGATTAAAGTCCAAATGCCCATCCTTAG30.19672131147540983No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG30.19672131147540983Clontech SMART CDS Primer II A (96% over 26bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG30.19672131147540983Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA30.19672131147540983Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30.19672131147540983Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG30.19672131147540983Clontech Universal Primer Mix Long (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT30.19672131147540983No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC30.19672131147540983Clontech SMART CDS Primer II A (96% over 26bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT30.19672131147540983Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.19672131147540983No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGGATCAACGCAGAGT20.13114754098360656Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTCTAATTGGTCGTTGTCGATTGCGGTATTTATTTTGATTGATGGACTT20.13114754098360656No Hit
TTCTTGAGTACTCTCCTTCAGCCCTAAGGTACCCTTTAGACTTCTCTTTC20.13114754098360656No Hit
AGAAAAGTTAAATCAAGAAATCATTGAACATGAAGAAACAATTGCACAAA20.13114754098360656No Hit
GTGCGCGCGTTAATTCAACGCATGCAAGAACTCGGCTATCCGATTAGCGA20.13114754098360656No Hit
CCCCGGACCCTTGGTATGACGACATGCCGCGCTGGATTCAGGGGGTGAGC20.13114754098360656No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT20.13114754098360656No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.13114754098360656Clontech Universal Primer Mix Long (96% over 26bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20.13114754098360656Clontech Universal Primer Mix Long (96% over 27bp)
GGTGAGCTCGTGGACCGCGGTGCTTACGAGGCTGCTAACCGTAAGGCGGT20.13114754098360656No Hit
CTCATCAAATCATTAAAAATACTGTAGAAAAACCTGATGAGCTCGAAAAA20.13114754098360656No Hit
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20.13114754098360656Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAGCTGGAGCGCAGCGGGGTGGAGCTGCGCTTGAACACCAGGGTTGGCGT20.13114754098360656No Hit
GTGGAGAACTCTCAGGTGGCCAGGCGGCGCTAGTAGGTCTGGCTCGGGCA20.13114754098360656No Hit
ACCAATAATACCGCGAGATTTAAACGTTTTTTAACTTTTGAATTTACCCT20.13114754098360656No Hit
GCAGGGATCGATGCCTTGCGCAGGATCGAACCGCTTGTCGTCAGGAGTCC20.13114754098360656No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG20.13114754098360656Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGGTAGCAGAACGTCGGGAAGATGACCGGGAAAACGCAGCCACGTCGACT20.13114754098360656No Hit
GACATGGATGAAGCTGGAAGCCAGCATCCTCAGCAAAGTAACACAGGAAC20.13114754098360656No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT20.13114754098360656Clontech Universal Primer Mix Long (96% over 25bp)
CCCCATGCTTTAACTGTGATGGGAAAAACCACCTGGATACTAGGCAAGTC20.13114754098360656No Hit
TTCTTAAATGACTGAGAATGTCAAGTTAAATCAAGTTAATTTGACTTCTT20.13114754098360656No Hit
CCTGGATGCCGTGGCCGGCCAGCTGGGTGAATAGCCCGTTGAGGCCGAGG20.13114754098360656No Hit
CTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGTCTTCTGCTTGA20.13114754098360656Illumina PCR Primer Index 8 (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT20.13114754098360656Clontech Universal Primer Mix Long (96% over 28bp)
GAATTATCATGAAATTGAAATGGATGGACTCATCATCGAATGGATTCGAA20.13114754098360656No Hit
GAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAA20.13114754098360656Illumina PCR Primer Index 9 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13114754098360656No Hit
TCGTTGACGGTAGTTTTGTAGACGGTCGTTTTCTCAGCCAGCGCGTCCCA20.13114754098360656No Hit
CACCAAAGCGGTGATCCAGGCCAAGTTGAACAGGCGAAAACGCTTGTGCC20.13114754098360656No Hit
GTGCAGGGCGCGGGACCTGCCATCCAAGACGACGGGCGGCTGCCAGGGTT20.13114754098360656No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT20.13114754098360656No Hit
AGTAGTAAGTGTCCTAATTTCAGAATTTTCCAAACAGTAAATAAAAACCA20.13114754098360656No Hit
GCTTTCGGTAGCCCGGCGTTGGCGAGCTCTTCGTCGCTGGGCAAGCGCAG20.13114754098360656No Hit
ATTCTGGGAGGAATGCCGGATGAACGGCGAGTCCATCATCGTTTGGTTTT20.13114754098360656No Hit
GAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAA20.13114754098360656RNA PCR Primer, Index 38 (96% over 28bp)
AGGACAGATAAAAATAAATGGAGAAAATGTCGTGATTGACTCTCCGGATA20.13114754098360656No Hit
GAGTTAAATAAAGTATTTCCAAATGCAAAAATTTTGCGGATGGATGTAGA20.13114754098360656No Hit
GGTCTCCGGGGCCGCCGCAGACCGGACCCCCCAGTGGACCGGTGAGCGAG20.13114754098360656No Hit
TCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATA20.13114754098360656No Hit
TGGCTGGGCCGATCAGGGCTGCTGCCCCTGTGGCAGCGCCGGAGCGTTGA20.13114754098360656No Hit
GTTGGCGACGATGTTGACCAGCTTGCCGGGGACCACGATGACCTTGCGGA20.13114754098360656No Hit
ATTCCATGCGCATGAGCACCATCGACAGGTCCTCGACGTCGATGACCGGG20.13114754098360656No Hit
TCGCCATCACGACGCCGTAATCGTTGTAGTTGGGGTAGGTTGCAGTAACA20.13114754098360656No Hit
GAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAA20.13114754098360656TruSeq Adapter, Index 18 (96% over 27bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG20.13114754098360656Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTGGAGCCGTCGTCGACGATCACGATCTCCTTGGCGACGGGCAGCGCCA20.13114754098360656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)