FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT352.2950819672131146Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT241.5737704918032787Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT231.5081967213114755Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT140.9180327868852459Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT110.7213114754098361Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC110.7213114754098361Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG100.6557377049180327Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC100.6557377049180327Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA100.6557377049180327Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC80.5245901639344263Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC80.5245901639344263Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC70.45901639344262296Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT60.39344262295081966Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG60.39344262295081966Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC60.39344262295081966Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.32786885245901637No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA50.32786885245901637Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.32786885245901637No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC40.26229508196721313Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.26229508196721313No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT40.26229508196721313Clontech SMART CDS Primer II A (96% over 26bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT40.26229508196721313Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA30.19672131147540983Clontech Universal Primer Mix Long (96% over 25bp)
CTTTTCACATTTGCATCTTGTCACGTTTTTAGTTTTTCCATGGCTGTATT30.19672131147540983No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG30.19672131147540983Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT30.19672131147540983Clontech Universal Primer Mix Long (96% over 25bp)
CTGTCGCTCCTAGCTCCAGATGAAACTGACTGAGTACGTTCAGTCTGTTT30.19672131147540983No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG30.19672131147540983Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG30.19672131147540983Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA30.19672131147540983Clontech Universal Primer Mix Long (96% over 25bp)
AGCAACAACGGTTGAAGAAATCGATCCGCGCCTGGAAGAACTGGGAGGGC20.13114754098360656No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20.13114754098360656Clontech Universal Primer Mix Long (96% over 25bp)
CTGGAAAGCCGTCAAAGGCGCCAGATCCACGATGCCCGAACCGAAGCACT20.13114754098360656No Hit
AAATTATTTAGGTATCAATGAGCATGTCGCTGATTTAAAACCGATAATTG20.13114754098360656No Hit
CCTGGAAGGAAGCCGCCGACAACCACGACTCGGTAGCCAACGAAGCCTTG20.13114754098360656No Hit
GAGCTACTGATGAAGCTCCACGTGGAGACCTTCCTCCTCGACCTACGTGA20.13114754098360656No Hit
ACCCCGATGCCGCAGCCATCGGCAAAATGACGCGAAAAACGCTCCAGGCA20.13114754098360656No Hit
TCAGGTCCTTAGCGGCCTGTACTGGGGTGCAATCCTCGTCTGGCTTATTG20.13114754098360656No Hit
TCCTAAAGCTGTGTCAGCATTTAATACACCCACAAATGTAACATTTGGAA20.13114754098360656No Hit
GCGCACTCGTATCTGAGCGTCAATGTCAAGTGCGGCAGTGGGCTCGTCGA20.13114754098360656No Hit
GAGCCCATCACCTAGGTTTTAAGCCCCACATGCATTAGCTATTTGTCCTG20.13114754098360656No Hit
ACCTTCAACAACAACGGCTCGCTGATCAAAAACCAAACGATGATGGACTC20.13114754098360656No Hit
TCTTTTAACTCATCTAGTTTATCTTGTTCATTTTTTGCATCTAATAATTG20.13114754098360656No Hit
GCAATGGTCCTCGCCGCATCGAGAAGTCGTCATCGCGCTTGACGCGCTGG20.13114754098360656No Hit
GGAATTCGGCAAGGGATCGACCAACCGGGTGTTGCGGATGCGCGGCTTCG20.13114754098360656No Hit
GTTCAACTGTGCACTCCACAATAGGGTCTCCTGAAGACAGATCCAAACAG20.13114754098360656No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT20.13114754098360656Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA20.13114754098360656Clontech Universal Primer Mix Long (96% over 25bp)
GGTCAAGGCCAGATGCTCGAGGCCCCCGACATCGGTTTGAAGGTGCGTGT20.13114754098360656No Hit
TCCTGGAGGAACGGCGGACGGGAGACTCCTTCCAAGCGGAGGACCTCCTC20.13114754098360656No Hit
CCATTCAATGAATCCATTCGATTCCATTGTATGACGATTCCATTCATTTC20.13114754098360656No Hit
ATGTGCATAAAGCATTTAAGAAGTCAAATTAACTTGATTTAACTTGACAT20.13114754098360656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13114754098360656No Hit
GAGCATTGACGCTGCGGATGGTCTCACCCTTCACGACTGTCGCATCATCG20.13114754098360656No Hit
GACCTAGCCGAGCTGCCGAGCGAGCTTCGAGAGGCGGCGTATGGCTGGCT20.13114754098360656No Hit
GTTCCCGTGCAGCCTTGCCGTTCGGCCGCCTGCCGTCAAGTCGCCTGGAA20.13114754098360656No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG20.13114754098360656Clontech Universal Primer Mix Long (96% over 25bp)
CATTAAGACTGAGAGCCCCTGCTTCAGAAACTCTCAGATTTGGAGATTTT20.13114754098360656No Hit
GCCGGTTGGGCGGCGGCGACGGATCAGATGGCTTGCCTATGTGCGCGCCT20.13114754098360656No Hit
CTGCTTTTCTTCTTCTGAAGCTGATTCGCTTTTTTTTTCTTGTTCTTGCT20.13114754098360656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13114754098360656No Hit
GTCTTAATATCTATAATTCCTGCTTTGTGTTGTTCATGTCCGAGCATAAT20.13114754098360656No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC20.13114754098360656Clontech Universal Primer Mix Long (96% over 25bp)
TCCTAGAAGAATACTAAAAGCAAATACTTCGATGCAGTTGTTAACTAGAG20.13114754098360656No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT20.13114754098360656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)