FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001322347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850311
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8553010.058672650359691Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT491285.777650765425826Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT392284.613370872539576Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC289423.403695824233722Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT282603.3234898760571134Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC252702.9718538275995487Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC237712.795565387252429Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT221632.6064581076806017Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC193162.2716394354536167Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA186402.192139111454515Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG171442.016203483196148Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA152871.7978128002577882Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC126931.4927479475156737Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC117931.3869043208896508Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA111121.306815976742627Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT100361.1802740409097376Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG94291.1088883949519646Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC93321.097480804082271Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG87131.0246839097694844Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC81300.9561207605217386Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT80890.9512989953087753Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT72580.8535700467240809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67890.7984137568489647No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG52480.6171859472592969Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA49060.5769653691414083Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47990.56438173797587No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA43380.5101662803374295Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT41060.48288214547383246Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG40850.4804124608525587Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT37570.4418383391488526Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37490.44089750691217683No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT34500.4057339020664204RNA PCR Primer, Index 31 (95% over 22bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA33020.3883285056879189Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32850.3863292371849829Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT31230.36727738439229884Clontech SMART CDS Primer II A (96% over 26bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA30700.36104437082432195Clontech Universal Primer Mix Long (96% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG27320.32129420882477117Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA25800.30341839632793177Clontech Universal Primer Mix Long (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT21230.24967335480782912RNA PCR Primer, Index 31 (95% over 24bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT18760.22062515950046513Clontech Universal Primer Mix Long (96% over 25bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC18530.21792026682002233Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG18050.21227527339996777Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT17430.20498382356573067Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17160.20180851476694997Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG16280.19145936016351664Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15830.1861671788322155Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14380.1691145945424674Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC13840.16276397694490605Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT13540.15923585605737195Clontech Universal Primer Mix Long (96% over 28bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC13520.15900064799820302Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC13140.15453169487399315Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG12520.14724024503975605Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG11900.13994879520551892Clontech Universal Primer Mix Long (96% over 25bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA11750.1381847347617519Clontech Universal Primer Mix Long (96% over 25bp)
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG11440.13453900984463332Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11130.13089328492751476Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10510.12360183509327763No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.12007371420574353No Hit
CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10060.1183096537619765Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9910.11654559331820945No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG9890.11631038525904051Clontech Universal Primer Mix Long (96% over 25bp)
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA9140.10749008304020528Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.10078665335389052Clontech SMART CDS Primer II A (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)