FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001322351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882360
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT764551.9692918740147745Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT418251.0773086473176108Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT348660.89806200352363Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC277530.7148487002750904Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT270420.6965350972089142Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC227790.5867307513986338Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC213460.5498202124480986Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT204470.5266641939438899Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC173200.44612040099321026Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA158960.4094416798030064Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG157930.40678865432365885Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA132720.3418539239019565Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA124450.3205524474803985Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC103890.2675949680091491Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT97620.251444997372732Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC96650.24894651706693868Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA94890.2444131919760146Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA84660.21806323988501838Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC78460.2020935719510813Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC78380.20188751171967564Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG78180.20137236114116155Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT77610.19990418199239637Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT76630.1973799441576773Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG73480.18926632254608022Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG44890.11562554734748968Clontech Universal Primer Mix Long (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT44880.11559978981856397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44790.11536797205823261No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT44020.11338464233095334Clontech SMART CDS Primer II A (96% over 26bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG42430.10928919523176624Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA41680.10735738056233837Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)