FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT613.530092592592592Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT251.4467592592592593Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT201.1574074074074074Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT170.9837962962962963Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC110.6365740740740741Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC110.6365740740740741Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA110.6365740740740741Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT100.5787037037037037Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC80.4629629629629629Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA80.4629629629629629Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA70.40509259259259256Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC70.40509259259259256Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT60.3472222222222222Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC60.3472222222222222Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC50.28935185185185186Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG50.28935185185185186Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA50.28935185185185186Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40.23148148148148145Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT40.23148148148148145Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40.23148148148148145Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC40.23148148148148145Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG40.23148148148148145Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC40.23148148148148145Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.23148148148148145No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT30.1736111111111111No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG30.1736111111111111Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG30.1736111111111111Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG30.1736111111111111Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1736111111111111No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC30.1736111111111111Clontech Universal Primer Mix Long (96% over 25bp)
CTTTTCATGCACGCGGTTTTGGGCCTATCGAACGCCATCCTCAGGGAGAT20.11574074074074073No Hit
GAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAA20.11574074074074073RNA PCR Primer, Index 26 (96% over 31bp)
GGGTGCGCGCCAGGAAGCCGGCTTGGATGAGGAAGGGCTCCACCACGTCG20.11574074074074073No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.11574074074074073Clontech Universal Primer Mix Long (96% over 26bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20.11574074074074073Clontech Universal Primer Mix Long (96% over 27bp)
GGCCAGGGCATTGACTGAAGCCTTGGAACGGGCCCACCGCGGTCAGCAGG20.11574074074074073No Hit
CCAGGTGTTCCAGTCCCTCGCCAGCTCCCCGCACGCGCGCCTCGAACGCT20.11574074074074073No Hit
CTAGCAACCTGGCTCGTTACGACGCCATGCGTTACGGCTTACGCGTCAAT20.11574074074074073No Hit
ACACAAGCCTGGAAAAATACTTTGACAGGCTCAACGGCACGACTGAATGG20.11574074074074073No Hit
TCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA20.11574074074074073RNA PCR Primer, Index 38 (96% over 28bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20.11574074074074073Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG20.11574074074074073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT20.11574074074074073Clontech Universal Primer Mix Long (96% over 25bp)
GAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAAAAA20.11574074074074073Illumina PCR Primer Index 1 (96% over 27bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC20.11574074074074073Clontech Universal Primer Mix Long (96% over 25bp)
ATATACAGAAGATTGAAGCTGGATGCCTTCCTTACACTATTTACAAAAAT20.11574074074074073No Hit
GCTCTACCCCAGCTTCTCGTCGCTGTCGCTGAAGCGTGTGTGGATCCTCA20.11574074074074073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11574074074074073No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA20.11574074074074073Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20.11574074074074073Clontech SMART CDS Primer II A (96% over 26bp)
GTCCTGGACCGGCGGGGCCTGGCTGGTCGCGGGCGGGCTGCGCTACGACC20.11574074074074073No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG20.11574074074074073Clontech Universal Primer Mix Long (96% over 25bp)
GAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA20.11574074074074073TruSeq Adapter, Index 27 (96% over 28bp)
GGCCCGGAATCGCGTGCTGCGCTACGCCCTGCTGCCGCTGATCGAGATCG20.11574074074074073No Hit
TAGGTTGATAAGTCAGCGTCTTAGTTGGCGTGATAGTTGCCAAGCTGCCT20.11574074074074073No Hit
ATGTCATCGTCATCGGTGTGGACCAGGAGAACGCCTGATGAGCAAATTCA20.11574074074074073No Hit
TCCTTCTCTAGTGTTCTGGAGTTCTGAAAATTGGATGCTTTATTTGAAAA20.11574074074074073No Hit
GCTCAAGCAGGCCCGGGCGAGTTCGGTGCCGGAACGAAATCAGCCCTGGC20.11574074074074073No Hit
GAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA20.11574074074074073Illumina PCR Primer Index 11 (96% over 29bp)
ATCCCAGGCGCCGCGCCAATTCGGCGTTATTGCCCAGCTCTGCATGGGCA20.11574074074074073No Hit
GTCATGGGCTGCTCCTCGCTCTGGATGCGGATCTGCAGCGGCACCACCTC20.11574074074074073No Hit
CTACAGGCGCAGCGATACGCCTTGTTTGGCGAACAAGCGGTAGACATCGT20.11574074074074073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11574074074074073No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT20.11574074074074073Clontech Universal Primer Mix Long (96% over 25bp)
GAATGGATCGATCGTTGTCGAGATAGGTAATGCATGGGAACTCGGGAAGC20.11574074074074073No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG20.11574074074074073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAA20.11574074074074073Illumina PCR Primer Index 12 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)