FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT281.6203703703703702Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT261.5046296296296295Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC191.099537037037037Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT170.9837962962962963Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC140.8101851851851851Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC110.6365740740740741Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.6365740740740741No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA90.5208333333333333Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT90.5208333333333333Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG90.5208333333333333Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC80.4629629629629629Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT80.4629629629629629Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC70.40509259259259256Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC60.3472222222222222Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG60.3472222222222222Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA60.3472222222222222Clontech Universal Primer Mix Long (96% over 25bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG50.28935185185185186Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.28935185185185186No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40.23148148148148145Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40.23148148148148145Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC40.23148148148148145Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT30.1736111111111111Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30.1736111111111111Clontech SMART CDS Primer II A (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1736111111111111No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT30.1736111111111111Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC30.1736111111111111Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA30.1736111111111111Clontech SMART CDS Primer II A (96% over 26bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC30.1736111111111111Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC30.1736111111111111Clontech Universal Primer Mix Long (96% over 25bp)
GCGCCGAATTGGCCTCGGTGATGTCGTTGCCGACCCGGAACGGCCGGTGC20.11574074074074073No Hit
GTGGAGAACCTGGCCATCGCCGTCAGCGAGGAGCTGGACACCGTCACCGA20.11574074074074073No Hit
TGATATAGTTGGCCGGCAGCGCGGGCGGATTGAAGTTGGCGGGGATGTCG20.11574074074074073No Hit
CAATTATTCCATAACTAGAATTTTTAAGTGAGGTGTCTTGAGTGACTGAG20.11574074074074073No Hit
CCCAGCCGACAAGGCATAGGTACCCAAGATGATGCGACGCTTGGCCTCGG20.11574074074074073No Hit
ACCCGCCACCATGTCCTGCTAATTTTTGTATTTTTAGTAGAGACGGGGTT20.11574074074074073No Hit
TGGCGCCACAGACGCCCCAGACGCTGTAGCAGCATGTCGGTCGGGGCAAA20.11574074074074073No Hit
CCGCATGCGCACCGCATTGGTCTCCTTGGCCATCTTCCGTGCGGTCTGCA20.11574074074074073No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG20.11574074074074073Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG20.11574074074074073Clontech SMART CDS Primer II A (96% over 26bp)
CACAGATGAAGGCCGCCCTTTTCGAGGAAGGCCAGCAGTGCTTTCAGCAC20.11574074074074073No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA20.11574074074074073Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGCATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20.11574074074074073Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGGTGTGGTGCCCGGGGCTGTGGTATTCGCGGGAGTCGTGGTGGTTGCGC20.11574074074074073No Hit
GCTTATATTCAAGGTATTGGCGGACAAACGGCTCCCCAATGTCTATCTGG20.11574074074074073No Hit
TCTTCCAGGAATTTTAAATGTTTAAGTTTTACATTTAAGTTTTTAATCAA20.11574074074074073No Hit
CTGGAAGACGGCGAGGCGGACCTGGAAACCCTGCTCAAGCTGGCCGCCGC20.11574074074074073No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG20.11574074074074073Clontech Universal Primer Mix Long (96% over 25bp)
GGTGCTGGTACATCACGGCTATTTCTGGAAGAACGAGCCAGTAGCGATTA20.11574074074074073No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC20.11574074074074073Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGCGGTATCAACGCAGAGTAAGCAGTGGTATC20.11574074074074073Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCTCCGACAGAACGGCAACGACGAGGCAGAGGCTGGTCGCGGTCCGCAA20.11574074074074073No Hit
GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT20.11574074074074073Clontech SMART CDS Primer II A (96% over 26bp)
GACTTAGCTGCTCATGAAAGGCGGTTTGGCTGATTTGATAGTCCTGCAAA20.11574074074074073No Hit
TTGTACCGGGGCGGGATTTCGGCGGATTTTTCGCCCTGACGCAACGCTGG20.11574074074074073No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG20.11574074074074073Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT20.11574074074074073Clontech SMART CDS Primer II A (96% over 26bp)
AAGAAGTACAGGAAGTTGAAATTCTTCGGCGCGTAGTATTTGGAGAGATG20.11574074074074073No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)