FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001322401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2942484
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2221827.550831202480625Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1404214.772192474113708Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC888773.0204752175372915Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT884963.0075269738085235Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT855212.9064219210707685Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC677152.3012869398780076Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT547461.8605368797247495Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC546931.8587356804658923Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA368321.2517315302309204Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC362731.2327339757837257Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC345941.1756733426587875Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA302671.0286207163743286Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC281020.9550434258945842Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC263470.8953999410022281Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG248790.8455101200210434Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT246410.8374217158020231Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG220150.7481773902593863Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA200220.6804455011480096Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC193670.6581853970998652Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT165960.5640132622641278Clontech Universal Primer Mix Long (96% over 25bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT157700.5359417417392924Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG143720.48843086317546675Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA130910.44489621693779813Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT126460.42977294014173056Clontech Universal Primer Mix Long (96% over 28bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT124030.42151461146432745Clontech Universal Primer Mix Long (96% over 27bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC118720.40346863398407606Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA113440.38552461117885434Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA112100.38097063569419576Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG111930.3803928925356943Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG95800.3255752622614091Clontech Universal Primer Mix Long (96% over 25bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT95340.3240119572442875Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT84090.2857789541081617Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT82980.2820066311320639Clontech Universal Primer Mix Long (96% over 28bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT76630.2604262249174507Clontech Universal Primer Mix Long (96% over 25bp)
GAGAAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73860.25101240992304463Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70840.24074897263672462Clontech Universal Primer Mix Long (95% over 24bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA69000.23449575256823826Clontech Universal Primer Mix Long (96% over 25bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68460.23266056841770422Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66700.22667922748263034Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGA63110.21447865137074665Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGAAAGCAGTGGTATCAACGCAGAGT62670.21298331613697816Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT54790.1862032214958518Clontech Universal Primer Mix Long (96% over 28bp)
GTGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54330.1846399164787302Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTAT53050.18028985034413103Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG52250.17757105901000653Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGT52150.17723121009324094Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC52120.17712925541821128Clontech Universal Primer Mix Long (96% over 28bp)
GCTTATTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50010.1699584432744579Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA48350.16431695125614956Clontech Universal Primer Mix Long (100% over 25bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC48250.163977102339384Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGT47810.16248176710561552Clontech SMART CDS Primer II A (95% over 24bp)
GTATTAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT46230.15711215422071964Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44740.15204840536091274Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG44240.15034916077708493Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT44120.14994134207696627Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC43270.14705262628445898Clontech Universal Primer Mix Long (96% over 28bp)
GCTTACTCTGCGTTGATACCACTGCTTTCTCTGCGTTGATACCACTGCTT40930.1391001616321448Clontech Universal Primer Mix Long (96% over 25bp)
GCATTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG40500.1376388112900529Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATC40270.1368571587814921Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCATCGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37160.12628785747008311Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36520.1241128244027835Clontech Universal Primer Mix Long (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAAT36120.12275342873572125Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35710.12136004817698244Clontech SMART CDS Primer II A (96% over 26bp)
ACGCTGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35590.12095222947686377Clontech Universal Primer Mix Long (96% over 25bp)
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG35220.11969478848483119Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34210.11626231442549899Clontech SMART CDS Primer II A (95% over 24bp)
GCGTCGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34030.115650586375321Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCT34030.115650586375321Clontech Universal Primer Mix Long (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAATAAGCAGTGGTATCAACGCAGAGT33160.11269390079946058Clontech Universal Primer Mix Long (95% over 24bp)
TATCTACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32410.11014503392371888Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGC32130.10919345695677529Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGAATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA32130.10919345695677529Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCCTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32100.10909150228174563Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGG31580.10732428791456469Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC31480.10698443899779915Clontech Universal Primer Mix Long (96% over 25bp)
CTGCATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30500.10365391961349663Clontech Universal Primer Mix Long (96% over 25bp)
TTCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30180.10256640307984682Clontech Universal Primer Mix Long (96% over 25bp)
GTTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30150.10246444840481715Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)