FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001322402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2942484
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2330287.919431337604554Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1779996.049276733535339Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1194114.05816989998926Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1154033.9219584541496233Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT846092.875427699861749Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT685672.330242067586434Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC581961.9777847560088688Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC481951.6379018543516293Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC412571.402114675899682Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC351701.195248640264484Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC318621.0828266185984359Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA316291.0749081388377983Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG303641.0319172508669545Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC302991.0297082329079785Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA285580.9705405364990939Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG249520.847991017113432Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC237720.8078888449350957Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA233900.7949066163146511Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT202170.6870725550249381Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT196810.6688566530863039Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG170410.5791365390601955Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG153120.5203766613514296Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG135440.4602913728672781Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT129310.4394586342695492Clontech Universal Primer Mix Long (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC126130.4286514387164042Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT111100.37757214652654014Clontech Universal Primer Mix Long (96% over 27bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT108320.36812434664045757Clontech Universal Primer Mix Long (96% over 28bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA106690.36258480929717885Clontech Universal Primer Mix Long (96% over 25bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT106650.3624488697304726Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA97680.33196442189660164Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA96180.3268666881451182Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT91960.3125250638576115Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT87810.2984213338118406Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA82860.28159881243194523Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75920.2580132976084152Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGT70760.24047709350331217Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC70460.23945754675301548Clontech Universal Primer Mix Long (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGA69190.23514146551009285Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGT67100.2280386231496926Clontech SMART CDS Primer II A (95% over 24bp)
GAGAAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66500.22599952964909917Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGAAAGCAGTGGTATCAACGCAGAGT65480.22253307069809045Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT64180.21811503478013816Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60650.2061183680183138Clontech Universal Primer Mix Long (95% over 24bp)
GCTTCCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55390.18824231499644517Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTTCTCTGCGTTGATACCACTGCTT53460.18168323090286984Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT51730.17580384464282559Clontech Universal Primer Mix Long (96% over 28bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT50680.17223543101678718Clontech Universal Primer Mix Long (96% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG50400.17128385404984361Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTAT50090.17023032240787037Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43760.14871788597661023Clontech SMART CDS Primer II A (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCT42200.14341624287506746Clontech Universal Primer Mix Long (96% over 28bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC38100.12948243728767939Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAAT37120.12615191790337688Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36220.12309327765248682Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTTCTCTGCGT35850.12183583666045422Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA35540.12078230501848099Clontech Universal Primer Mix Long (100% over 25bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC35420.12037448631836231Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAATAAGCAGTGGTATCAACGCAGAGT35350.1201365920766264Clontech Universal Primer Mix Long (95% over 24bp)
GTATCATCGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34990.1189131359762704Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTTCTCTGCGTTGATACCACTGC34840.11840336260112204Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATC33260.11303374971622616Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTATTCTGCGTTGATACCACTGCTT32910.11184427850754668Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGG32720.11119856556569212Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG32420.11017901881539544Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGT32230.10953330587354086Clontech SMART CDS Primer II A (95% over 24bp)
ACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC32200.10943135119851119Clontech Universal Primer Mix Long (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAGGAAGCAGTGGTATCAACGCAGAGT30950.10518323973894164Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)