FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001322427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948522
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT913334.687296319979964Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT572642.938842876806113Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT337761.733416404844287Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC321641.6506870335567165Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT270091.3861275366662527Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC261291.3409651007276284Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC217081.114075181085972Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT187720.9633968720907435Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA159140.8167215971900753Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC143750.7377386552474132Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTG137250.7043800377927475Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA134420.6898562089624855Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC113460.5822874979086713Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG98240.5041770121148235Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT93460.47964559804816165Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC93360.47913238854885903Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAA92120.4727685907575075No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC84950.4359714696575148Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG82070.42119103607760144Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGAT70070.3596058961612956Clontech Universal Primer Mix Long (95% over 21bp)
CAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGC68240.35021416232405894Clontech Universal Primer Mix Long (95% over 22bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA67880.3483666081265698Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTG67760.3477507567274067Clontech Universal Primer Mix Long (95% over 22bp)
GCTTACTCTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTAC66340.34046318183731056Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC63520.32599067395697867Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGC60770.3118774127261586No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGTAA60440.3101838213784602Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCT59980.30782305768166845Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAAC59570.305718898734528Clontech Universal Primer Mix Long (95% over 22bp)
GCTTACTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC56920.2921188470030105Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT56910.29206752605308023Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAG56470.28980940425614904No Hit
GTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAG56430.28960412045642797Clontech Universal Primer Mix Long (95% over 22bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA55880.28678146821026396Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTG47290.2426967722201751Clontech Universal Primer Mix Long (95% over 21bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG45580.23392088978210152Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG44390.2278136967404012Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA43610.22381066264584132Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATAC42730.21929441905197888Clontech Universal Primer Mix Long (95% over 21bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39150.20092151897694766Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACG39070.2005109513775056No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA39030.2003056675777846Clontech Universal Primer Mix Long (96% over 25bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38430.1972264105819693Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36770.188707132893547No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35330.1813169161035903Clontech Universal Primer Mix Long (96% over 27bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC34920.17921275715644985Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34780.1784942638574263Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG34600.17757048675868173Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT33800.17346481076426132Clontech Universal Primer Mix Long (96% over 28bp)
GCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGT32220.16535610067528106No Hit
CTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTAC31660.16248212747918678Clontech Universal Primer Mix Long (95% over 21bp)
CAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTA29500.15139680229425176Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCA29250.1501137785459954No Hit
GATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCAC29000.148830754797739Clontech Universal Primer Mix Long (95% over 21bp)
GCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTC28580.1466752749006683Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTT28500.14626470730122626Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT28490.14621338635129602Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATC27800.14267224080610844No Hit
ACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTA27780.1425695989062479Clontech Universal Primer Mix Long (95% over 22bp)
ATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACT27660.14195374750708487Clontech Universal Primer Mix Long (95% over 21bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT26290.13492277736663996Clontech Universal Primer Mix Long (96% over 25bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24560.12604425302870587Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCA24490.12568500637919408Clontech Universal Primer Mix Long (95% over 21bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24040.12337556363233261Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT23540.12080951613581986Clontech Universal Primer Mix Long (96% over 28bp)
GTGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22850.11726837059063229Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA21820.11198231274781605Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20880.10715814345437209No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAA20700.1062343663556275No Hit
GAATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20590.1056698359063947Clontech Universal Primer Mix Long (95% over 24bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA20540.1054132311567434Clontech Universal Primer Mix Long (96% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG20330.10433549120820806Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG19600.10058906186329947Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)