FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001322428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948522
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT914284.692171810223338Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT783064.018738305238535Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC413802.123660908113945Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT404212.07444411713083Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT306221.5715501287642635Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT234491.2034249549145455Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC211391.0848735605756568Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC184920.9490270061102722Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTG179430.9208518045985624Clontech Universal Primer Mix Long (95% over 21bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC133470.6849807187191111Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA131570.6752297382323628Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA124090.6368416676845322Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC123930.636020532485648Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC116850.5996852999350276Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG115720.593886032592909Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG102710.5271174767336474Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC99540.5108487356057565Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGAT97950.502688704566846Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAA91420.4691761242623897No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTAC85750.4400771456519351Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAG78880.40481965304985007No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA77500.3977373619594749Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC76210.391116959418472Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC74400.38182786748109593Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG73900.3792618199845832Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT72410.37161499844497525Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCT70470.36165873415850575Clontech Universal Primer Mix Long (95% over 21bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT66280.340155256137729Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATAC64070.3288133262031427Clontech Universal Primer Mix Long (95% over 21bp)
GAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGC62740.3219876398624188No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGTAA61650.316393656320021Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTG60900.3125445850752519Clontech Universal Primer Mix Long (95% over 22bp)
CAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGC60190.30890079763020384Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAAC58940.30248567888892197Clontech Universal Primer Mix Long (95% over 22bp)
TACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTG51980.2667662977374646Clontech Universal Primer Mix Long (95% over 21bp)
GTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAG49700.2550651211533665Clontech Universal Primer Mix Long (95% over 22bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG46590.23910430572505725Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG45850.23530655543021842Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA43840.22499104449423715Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGT43630.22391330454570182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39170.2010241608768082No Hit
GCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTC39160.20097283992687792Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTAC37670.19332601838726995Clontech Universal Primer Mix Long (95% over 21bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC36980.18978487284208237Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA36410.18685957869605782Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATC35290.1811116323038693No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT34560.1773652029589607Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTT34350.17628746301042533Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACG33120.169974986169004No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT32060.16453496547639698Clontech Universal Primer Mix Long (96% over 28bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA31930.16386779312730365Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCAC31830.16335458362800112Clontech Universal Primer Mix Long (95% over 21bp)
ATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACT31060.1594028704833715Clontech Universal Primer Mix Long (95% over 21bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT30900.1585817352844874Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT29410.15093491374487944Clontech Universal Primer Mix Long (96% over 25bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28820.1479069776989944Clontech Universal Primer Mix Long (96% over 27bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27880.14308280840555046Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27340.14031147710931668Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCA26800.13754014581308294No Hit
TGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCA25530.1310223851719406Clontech Universal Primer Mix Long (95% over 21bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA25150.1290721890745909Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTGCGTTG25040.1285076586253581Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTA24630.12640349967821762Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTA24360.12501783403010078Clontech Universal Primer Mix Long (95% over 22bp)
GCAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG23660.12142536753498293Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTA22350.11470232309411954Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.10777399485353514No Hit
GCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTCTGCGTTG20190.1036169979091845Clontech Universal Primer Mix Long (95% over 21bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG20120.10325775125967272Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19930.10228265321099789Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGAGTAA19780.10151283896204404Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)