FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001322429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565458
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3242779.094960591318141Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1909915.356703122011253Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1298693.6424212541558476Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1254423.518257682463235Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1239623.4767482887191488Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1041182.9201858498964226Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC837912.350076764331539Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT814422.2841946252066356Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC564121.58218102695362Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC543991.5257226420841306Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA524371.4706946484855523Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA451951.267579088016182Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC432901.214149767014504Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC403141.130682229323694Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG367771.0314803876528624Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT349710.9808277085300122Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG323950.9085789259051713Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC296150.8306085781966861Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA289270.8113123194832192Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT245590.6888035141628368Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG178250.4999357726272473Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA171790.4818174831957072Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG164060.460137239030722Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA160830.45107809431495194Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT159080.4461698889735905Clontech Universal Primer Mix Long (96% over 28bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA152290.427126052249108Clontech Universal Primer Mix Long (96% over 25bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT152130.4266773020464692Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC151230.42415308215662617Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT143420.4022484628903215Clontech Universal Primer Mix Long (96% over 27bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT137470.3855605647296925Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG133970.3757441540469696Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT122110.34248054527637123Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT113550.3184724094351974Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA106250.29799818143980383Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT94750.26574426062514267Clontech Universal Primer Mix Long (96% over 28bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT89560.2511879259270478Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT77750.2180645515947741Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77450.21722314496482642Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG75040.21046384503758003Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC68280.19150414897609228Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT66430.18631547475808158Clontech Universal Primer Mix Long (96% over 28bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG65280.18309008267661545Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTAT64430.18070609722509703Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGT64210.18008906569646874Clontech Universal Primer Mix Long (96% over 28bp)
GTATTAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT63480.17804164289692936Clontech Universal Primer Mix Long (96% over 25bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT61940.17372242219653128Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC61610.17279687490358883Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGT61360.17209570271196575Clontech SMART CDS Primer II A (95% over 24bp)
GCATTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG59650.16729968492126399Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC57660.16171835427594436Clontech Universal Primer Mix Long (96% over 28bp)
ACGCTGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC57650.16169030738827941Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATC53360.1496581925800276Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC50730.14228186112415292Clontech Universal Primer Mix Long (96% over 28bp)
GAGAAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49910.13998201633562923Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49640.13922475036867635Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGA48190.13515795165726255Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA48110.13493357655594318Clontech Universal Primer Mix Long (100% over 25bp)
TATCTACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC45020.12626708826748204Clontech Universal Primer Mix Long (96% over 25bp)
CTGCATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC44750.12550982230052915Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGAAAGCAGTGGTATCAACGCAGAGT44510.12483669699657099Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTGGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44230.12405138414195314Clontech Universal Primer Mix Long (96% over 25bp)
TTCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT43600.12228443021906302Clontech Universal Primer Mix Long (96% over 25bp)
GCGTAGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42640.11959192900323043Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42280.11858224104729322Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTCGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42150.11821763150764923Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGC41780.1171798966640471Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41150.11541294274115695Clontech Universal Primer Mix Long (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCT40710.11417887968390035Clontech Universal Primer Mix Long (96% over 28bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC39820.11168270668172223Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39770.11154247224339763Clontech SMART CDS Primer II A (95% over 24bp)
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG38970.1092987212302038Clontech Universal Primer Mix Long (96% over 28bp)
CTGCCTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38750.1086816897015755Clontech Universal Primer Mix Long (96% over 25bp)
GTTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37620.10551239139543925Clontech Universal Primer Mix Long (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT37190.10430637522584757No Hit
GGGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36130.10133340513336576Clontech Universal Primer Mix Long (96% over 27bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)