FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001322445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2462785
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1352985.493699206386267Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT745893.028644400546536Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT536492.1783874759672486Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT501452.0361095264101414Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC484861.968746764333874Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC427521.7359209187972153Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC353041.4334990671130448Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT349081.417419709800084Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC237570.964639625464667Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC225330.9149397937700612Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA220320.8945969705029063Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA208510.846643129627637Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC185540.7533747363249329Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC172180.69912720761252Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG150720.6119900843963237Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT138620.5628587148289437Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG127340.5170569091496009Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT122760.4984600767017827Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC121680.49407479743461163Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA121240.492288202177616Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG83270.3381131523864243Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA65160.26457851578599023Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG62960.25564553950101204Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG61640.25028575373002515Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA59290.2407437108801621Clontech Universal Primer Mix Long (95% over 24bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA55960.22722243313971785Clontech Universal Primer Mix Long (96% over 25bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55730.22628853107356106Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT53160.21585319059520014Clontech Universal Primer Mix Long (96% over 25bp)
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT53030.21532533290563324Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA51280.2082195563153097Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT45820.18604953335350022Clontech Universal Primer Mix Long (96% over 28bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT45530.18487200466138945Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC44340.18004007657996943Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43370.17610144612704723Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT41530.16863022959779275No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA40680.16517885239677846Clontech Universal Primer Mix Long (96% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA37850.15368779653928377Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37640.15283510334844494Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36960.1500740015876335No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG33290.13517217296678355Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33280.13513156852912456Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC28620.11620990058003439Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT28180.11442330532303875Clontech Universal Primer Mix Long (96% over 25bp)
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27400.1112561591856374Clontech Universal Primer Mix Long (96% over 25bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27280.11076890593372948Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26900.10922593730268781Clontech Universal Primer Mix Long (96% over 27bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG25840.10492186691083469No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24810.10073960983195854Clontech SMARTer II A Oligonucleotide (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)