FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001322459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775634
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT830002.9903077999476877Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT429651.5479346340331614Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC318121.1461165268907934Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT303221.0924350977110096Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT302871.0911741245423567Clontech Universal Primer Mix Long (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT298421.075141751398059No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC265980.9582675525663686Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT240160.8652437605246225Clontech Universal Primer Mix Long (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT217930.7851539504127706RNA PCR Primer, Index 39 (95% over 23bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC192740.6943999100745991Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG158400.5706804283273659RNA PCR Primer, Index 39 (96% over 30bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC135050.4865555040758256Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC132470.4772603304326147Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA119890.4319373519707569Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC111650.4022504408001919Clontech Universal Primer Mix Long (96% over 25bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT108580.39118990472086734RNA PCR Primer, Index 39 (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC106280.3829035096125786Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA105800.38117417498128353Clontech Universal Primer Mix Long (96% over 25bp)
ATGTTAAGCAGTGGTATCAACGCAGAGTACCCATGTTAAGCAGTGGTATC90850.32731260677740653Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG87350.31470287509088013Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC82590.29755363999720424Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT80720.29081644049611727Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT78640.28332265709383875Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA73120.26343530883394567Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG69790.25143804982933626Clontech Universal Primer Mix Long (96% over 25bp)
GCTTAACATGGGTACTCTGCGTTGATACCACTGCTTAACATGGGTACTCT58260.2098979908734365Clontech SMART CDS Primer II A (96% over 26bp)
GTACCCATGTTAAGCAGTGGTATCAACGCAGAGTACCCATGTTAAGCAGT50420.18165219189561738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG48710.17549143727162875Clontech Universal Primer Mix Long (96% over 25bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT44280.1595311197369682RNA PCR Primer, Index 39 (96% over 28bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG40060.14432738610349924Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTAACATGGGTACTCTGCGTTGATACCACTGCTT38730.1395356880626192Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG38470.13859896513733438Clontech Universal Primer Mix Long (96% over 25bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37720.13589687977593587Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA36610.13189779344106609Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA34950.1259171778411707Clontech Universal Primer Mix Long (96% over 25bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34530.12440401003878754Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACCCATGTTAAGCAGTGGTATCAACGCAGAGTACCCATGTTAAGCA34110.12289084223640435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT33640.12119753540992798Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA33580.1209813685810161Clontech Universal Primer Mix Long (96% over 25bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA31920.11500075298112071RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACCCATGTTAAGCAGTGGTATCAACGCAGAGTACC30910.11136194469443739Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGTACTCTGCGTTGATACCACTGCTTAACATGGGTACTCTGCGTTG29250.10538132909454201Clontech SMART CDS Primer II A (96% over 26bp)
ATGTTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG28840.10390418909697749Clontech Universal Primer Mix Long (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT28790.10372405007288425Clontech Universal Primer Mix Long (96% over 28bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG28200.10159840958858408Illumina PCR Primer Index 11 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)