FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001322529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941485
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT973995.01672688689328Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT531652.738367795785185Clontech Universal Primer Mix Long (96% over 25bp)
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG484712.4965941019374345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT366861.8895845190665908Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT360571.8571866380631321Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC330181.7006569713389492Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC323811.6678470346152559Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC270161.391512167232814Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC252001.2979755187395214Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT238231.227050427894112Clontech Universal Primer Mix Long (96% over 25bp)
CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC173170.8919461134131864Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC164670.848165193138242Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC161700.8328676245245263Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA156420.8056719469890317Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA148210.7633847286999385Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC129490.6669636901650026Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC121370.6251400345611735Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG110470.5689974426791863Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT97490.502141402071095Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA93080.4794268304931534Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC91910.4734005155847199Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG91170.4695890001725483Clontech Universal Primer Mix Long (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT89670.4618629554181465No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA87910.4527977295729815Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC87230.449295255950986Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT81950.4220995784154912Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGC69830.35967313679992374Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTA65070.335155821445955Clontech SMART CDS Primer II A (96% over 26bp)
CATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAG59440.30615740013443316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT56690.291992984751363TruSeq Adapter, Index 3 (95% over 21bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG56590.2914779151010695Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCA56400.29049928276551196Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT49470.2548049560001751Clontech SMART CDS Primer II A (96% over 26bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG49060.25269317043397194Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA48740.25104494755303286Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA48620.2504268639726807Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44930.231420793876852Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGT44910.23131777994679328Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG41560.21406294666196238Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA41550.214011439696933Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA40060.20633690190756046Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTT40020.20613087404744304Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGAT39340.20262840042544755Clontech SMART CDS Primer II A (96% over 26bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT35690.18382835818973622Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT35280.18171657262353302Clontech Universal Primer Mix Long (96% over 28bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33010.17002449156187147Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC32600.16791270599566827Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT30850.15889898711553269Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA30670.15797186174500447Clontech Universal Primer Mix Long (96% over 25bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29000.14937019858510367Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGT27900.1437044324318756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCA27600.14215922348099522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAG26840.13824469413876492Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACT25540.13154878868494993Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT25080.1291794682936RNA PCR Primer, Index 3 (95% over 23bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCA24730.1273767245175729Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG24610.12675864093722072Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGC23890.12305013945510782Clontech SMART CDS Primer II A (96% over 26bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG23450.12078383299381659Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23060.11877506135767209Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATAAGCAGTGGTATCAACGCAGAGTACACAAGCAGTGGTATCAACG22880.11784793598714387Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG22720.11702382454667432TruSeq Adapter, Index 3 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.1119761419737984No Hit
TATTTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC21210.10924627282724306Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT21090.1086281892468909Clontech Universal Primer Mix Long (96% over 25bp)
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT20810.10718599422606923No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20310.10461064597460193Clontech Universal Primer Mix Long (96% over 27bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC20280.10445612507951388Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG19830.1021383116531933Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19750.10172625593295853Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACA19530.10059310270231292Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)