FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001322530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941485
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1053945.428525072302902Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT670473.4533874843225676Clontech Universal Primer Mix Long (96% over 25bp)
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG503762.5947148703183385Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT486982.508286182999096Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC424072.1842558659994795Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT356121.8342660386250729Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC315081.6228814541446368Clontech SMART CDS Primer II A (96% over 26bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT293821.5133776464922468Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC279591.4400832352554875Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC225921.163645353942987Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC190490.9811561768440138Clontech Universal Primer Mix Long (96% over 25bp)
CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC188600.9714213604534674Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC162470.836833660831786Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC147010.7572038928964169Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA145990.7519501824634236Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC142760.7353134327589449Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG136310.7020914403150166Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA129610.6675817737453548Clontech Universal Primer Mix Long (96% over 25bp)
TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC119810.6171049480165955Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA116030.5976353152355027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG107980.5561722083868791Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC106410.5480856148772718Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA100580.518057054265163Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT95710.49297316229587146Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG84670.4361094729034734Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT81870.42168752269525644Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTA79440.40917133019312535Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGC77560.3994880207676083Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCA59750.3077541160503429Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT58810.30291246133758437Clontech SMART CDS Primer II A (96% over 26bp)
CATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAG57800.29771025786962046Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG56400.29049928276551196Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG54320.279785834039408Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGAT47030.24223725653301467Clontech SMART CDS Primer II A (96% over 26bp)
GTACATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA47010.24213424260295596Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGT46140.23765313664540288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA44610.22977257099591292Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTT44260.22796982721988582Clontech SMART CDS Primer II A (96% over 26bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT40640.2093243058792625Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT38060.19603550890169125Clontech Universal Primer Mix Long (96% over 28bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT36590.18846398504237735Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC35800.18439493480505903Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA34840.1794502661622418Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA34350.17692642487580382Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA31790.16374064182829123Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAG29830.1536452766825394Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACT29210.15045184485071994Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGT27950.14396196725702234Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCA27520.14174716776076046Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCA26400.1359783876774737Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG26070.13427865783150528Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGC25880.1333000254959477Clontech SMART CDS Primer II A (96% over 26bp)
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24860.12804631506295439Clontech Universal Primer Mix Long (96% over 25bp)
GTACATAAGCAGTGGTATCAACGCAGAGTACACAAGCAGTGGTATCAACG24290.12511041805628167Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT24250.12490439019616428Clontech Universal Primer Mix Long (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22990.11841451260246667No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT22440.11558162952585262No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT20940.10785558477145073Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATA20030.10316845095378023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACACAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG19650.10121118628266508Clontech Universal Primer Mix Long (96% over 25bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19500.10043858180722488Clontech Universal Primer Mix Long (96% over 27bp)
CCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTT19450.10018104698207815Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)