FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001322541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321816
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34604714.904152611576455Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1659517.1474656045095735Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1116034.806711642955342Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1012804.362102767833455Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC989554.261965633796994Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT950334.093046132854628Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT768233.3087462572400224Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC687662.961733401785499Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC593122.55455212643896Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA509962.19638420960145Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA477372.0560199430101265Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC474182.0422806975229735Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC395461.7032357430562972Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC340451.4663091304392768Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT327011.408423406505942Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG320091.3786191498378855Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG317651.3681101344809408Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC227080.9780275439569716Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA199370.8586813080795378Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT190570.8207799412184256Clontech Universal Primer Mix Long (96% over 25bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT179230.7719388616496742Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG173430.7469584153093958Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA158180.6812770693284911Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA146660.6316607345284898Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG141510.6094798209677252Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG134350.5786418906580022Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA125710.5414296395580012Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT119520.5147694735500143Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG106640.45929565478056833Clontech SMART CDS Primer II A (96% over 26bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT103060.4438766896257068Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT91380.3935712390645943Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT91000.39193458913195534Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC90400.3893504050277886Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA88910.3829330145024412Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA88240.38004734225278836Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC79160.3409400228097317Clontech SMART CDS Primer II A (96% over 26bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66930.28826573681979967Clontech Universal Primer Mix Long (96% over 27bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC62910.2709517033218825Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT62340.2684967284229241Clontech Universal Primer Mix Long (96% over 28bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59370.25570501710729876Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG58590.25234557777188205Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA58170.25053664889896526Clontech Universal Primer Mix Long (96% over 25bp)
GTGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56200.24205191109028448Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATTAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT52600.22654680646528408Clontech Universal Primer Mix Long (96% over 25bp)
ACGCTGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC49590.21358281620938094Clontech Universal Primer Mix Long (96% over 25bp)
GCATTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG48210.20763919276979742Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCTACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC47900.2063040309826446Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC46890.20195398774063059Clontech SMART CDS Primer II A (96% over 26bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG46450.200058919397575Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA43710.1882578119885469Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43320.18657809232083852Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT43280.18640581338056073Clontech Universal Primer Mix Long (96% over 25bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC43020.18528600026875514Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AAGCTGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC41300.17787800583681052Clontech Universal Primer Mix Long (96% over 25bp)
GCGTGGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40680.17520768226250485Clontech Universal Primer Mix Long (96% over 25bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT40020.17236507974792145Clontech SMART CDS Primer II A (96% over 26bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG38980.1678858273006991Clontech SMART CDS Primer II A (96% over 26bp)
GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38180.16444024849514347Clontech Universal Primer Mix Long (100% over 24bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38020.16375113273403233Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA37130.15991792631285168Clontech SMART CDS Primer II A (96% over 26bp)
CTGCATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36710.15810899743993495Clontech Universal Primer Mix Long (96% over 25bp)
CTGCCTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35810.15423272128368484Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34360.1479876096986152Clontech SMART CDS Primer II A (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC33390.143809845396879Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC32450.13976129030035112Clontech Universal Primer Mix Long (96% over 25bp)
GCGTAGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31370.13510975891285099Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT31300.13480827076736487Clontech SMART CDS Primer II A (96% over 26bp)
TGCTGACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30300.13050129726042028Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC30220.13015673937986474Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCTAACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29980.129123065738198Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC29160.12559134746250347Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG28510.12279181468298953Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG28260.12171507130625339Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG28060.12085367660486447Clontech Universal Primer Mix Long (96% over 25bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT27570.11874325958646163Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA26900.11585758733680877Clontech SMART CDS Primer II A (96% over 26bp)
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG26040.11215359012083645Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT25530.10995703363229471Clontech Universal Primer Mix Long (96% over 28bp)
GCGTCGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24110.10384113125243345Clontech Universal Primer Mix Long (96% over 25bp)
AGATTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG23950.1031520154913223Clontech Universal Primer Mix Long (95% over 24bp)
TGCTCACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23700.10207527211458618Clontech Universal Primer Mix Long (96% over 25bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA23530.1013430866184056Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)