FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001322548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763766
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT545567.143025481626572Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT350844.593553522937654Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT281643.6875168572573274Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC208912.7352618472149848Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT180752.366562533550852Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT157912.0675180618147446Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC144011.8855251477546786Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC132471.7344317500386244Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC120431.5767918446225677Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC98771.2931971310584656Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA83231.0897316717423924Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC82621.0817449323483894Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC81921.0725798215683862Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG73810.9663954666743478Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA71270.933139207558336Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG64280.8416190299123031Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC58510.7660723310542757Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA56660.7418502525642671Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT53090.6951081875862503Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG50470.6608044872382378Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT42950.5623450114302024Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35260.4616597230041662Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33260.43547369220415677No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG30820.40352673462814526Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27290.35730839026612865Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG24860.3254923628441172Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA23700.3103044649801117Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT22460.29406912588410583Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21750.2847730849501025Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT21040.2754770440160992Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA18240.23881660089608595Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.22899683934608245No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA17490.22899683934608245Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.20215615777607276No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA14220.18618267898806704Clontech Universal Primer Mix Long (96% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG13880.1817310537520654Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13290.17400617466606264Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT13120.17178036204806185Clontech SMART CDS Primer II A (96% over 26bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT11670.152795489718055Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11050.14467782017005207Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG10790.14127363616605085Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC9690.12687131922604566Clontech SMART CDS Primer II A (96% over 26bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC8210.1074936564340387Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC7640.100030637656036Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)