FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001322585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028958
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT554505.388946876354526Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT301732.932384023448965Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT234582.279782070793949Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT201801.9612073573459752Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC187751.8246614536259012Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC176491.7152303592566462Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC166121.6144487918846058Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT152931.4862608580719523Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC109551.0646693062301862Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC101160.9831305067845335Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC94560.9189879470299079Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA92470.8986761364409431Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA91530.8895406809607389Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC83970.8160682943327132Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG67010.6512413529026452Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG62680.6091599462757469Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT57560.5594008696176132Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC54400.5286901894926712Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA54250.5272324040437024Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53900.5238309046627754Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT52290.5081840075105106Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG38570.3748452317781678Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT35660.34656419406817385Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34820.33840059555394875Clontech Universal Primer Mix Long (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT30180.2933064323325151No Hit
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28810.27999199189860036Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA28170.2737721073163336Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG25980.2524884397613897Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG25210.24500514112335003Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT24890.24189519883221666Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA23260.2260539302867561Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT21250.2065196052705747Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19340.18795713722037244No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA18270.17755826768439528Clontech Universal Primer Mix Long (96% over 25bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18160.17648922502181819Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA17620.17124119740553065Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT16780.16307759889130558Clontech SMART CDS Primer II A (96% over 26bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG16460.15996765660017223Clontech SMART CDS Primer II A (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT16210.15753801418522428RNA PCR Primer, Index 26 (100% over 22bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA15730.15287310074852425Clontech Universal Primer Mix Long (96% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG15600.1516096866927513Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC14560.1415023742465679Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG14460.1405305172805887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCTGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14080.13683746080986786Clontech Universal Primer Mix Long (96% over 25bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT12510.12157930644399478Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG11920.11584535034471766Clontech SMART CDS Primer II A (96% over 26bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC11750.11419319350255307Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG11370.1105001370318322Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG11290.10972265145904887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.10505773802234881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10780.10476618093255506No Hit
GTATTAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10540.10243372421420505Clontech Universal Primer Mix Long (96% over 25bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10450.10155905294482379Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)