FastQCFastQC Report
Thu 8 Sep 2022
EGAF00001322595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4344200
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57136813.152433129229776Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3043507.005892914690852Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2293165.278670411122876Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2292775.277772662400442Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2036354.687514386998757Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1572123.6188941577275444Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1554573.5784954652179914Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1274982.9349017080244924Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC985202.26785138805764Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC922802.1242115924681184Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA851901.9610054785691267Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC782781.801896781916118Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC737731.6981952948759265Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA653481.5042585516320612Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG584671.345863450117398Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT583011.3420422632475484Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG581821.3393029786842225Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC490381.1288154320703467Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA442441.0184613968049352Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT403280.928318217393306Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA311500.7170480180470512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT285380.6569218728419501Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG271840.6257538787348649Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA267850.6165692187284194Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT244270.5622899498181484Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG233310.5370609087979374Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG230780.5312370517011188Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA226460.5212927581603056Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT214830.4945214308733484Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA210020.4834491966299894Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT188850.43471755444040333Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG185090.42606233598821414Clontech SMART CDS Primer II A (96% over 26bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT183220.42175774596013077Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA181390.4175452327240919Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC154090.3547028221536762Clontech SMART CDS Primer II A (96% over 26bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA144120.3317526817365683Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC120750.2779568159845311Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT119030.2739975139266148Clontech Universal Primer Mix Long (96% over 28bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT108380.24948206804474932Clontech Universal Primer Mix Long (96% over 27bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC106510.2451774780166659Clontech SMART CDS Primer II A (96% over 26bp)
GTATTAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT104860.2413793103448276Clontech Universal Primer Mix Long (96% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG103670.23864002578150179Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT96250.22155978085723493Clontech Universal Primer Mix Long (96% over 25bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC95170.21907370747203167Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC94470.2174623636112518Clontech SMART CDS Primer II A (96% over 26bp)
GCGTGGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT91380.21034943142580914Clontech Universal Primer Mix Long (96% over 25bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90810.20903733713917408Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA89690.20645918696192625Clontech SMART CDS Primer II A (96% over 26bp)
GCATTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG89240.20542332305142486Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCTGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC86950.20015192670687354Clontech Universal Primer Mix Long (96% over 25bp)
GTGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT85770.19743566134155888Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG84440.19437410800607707Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG83380.1919340730168961Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT80960.18636342709819992Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT80680.18571888955388793Clontech SMART CDS Primer II A (96% over 26bp)
CTGCATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC80280.18479812163344228Clontech Universal Primer Mix Long (96% over 25bp)
GCGTAGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT75460.1737028681920722Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG74330.17110169881681322Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA73630.16949035495603332Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC73140.16836241425348739Clontech Universal Primer Mix Long (96% over 25bp)
TATCTACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC68790.15834906311864094Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC68080.15671470005984992Clontech SMART CDS Primer II A (96% over 26bp)
CTGCCTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC67100.15445881865475808Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA66030.15199576446756596Clontech Universal Primer Mix Long (96% over 25bp)
TGCTAACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT64030.1473919248653377Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63000.14502094747019012Clontech Universal Primer Mix Long (100% over 24bp)
TGCTGACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT62390.14361677639151052Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC59580.1371483817503798Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGCTGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC58900.13558307628562222Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT58640.13498457713733253Clontech Universal Primer Mix Long (96% over 28bp)
CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT58230.13404079001887573Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG56460.12996639197090373Clontech Universal Primer Mix Long (96% over 25bp)
GCGTCGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52200.12016021361815755Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG49300.11348464619492657Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA48140.11081441922563419Clontech Universal Primer Mix Long (96% over 25bp)
TACCTCTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC46970.10812117305833065Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA46760.10763776990009669Clontech SMART CDS Primer II A (96% over 26bp)
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG45250.10416187100041434Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTAT44450.10232033515952305Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGT44350.10209014317941163Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC44340.1020671239814005Clontech Universal Primer Mix Long (96% over 28bp)
GTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT44080.10146862483311081Clontech Universal Primer Mix Long (96% over 28bp)
ATACTACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC44050.10139956723907739Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)