FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001322617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3132334
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2362737.543033405760688Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1081643.453143885677581Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT914072.918175392534768Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC885442.826773900867532Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT834262.6633813635455224Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT709422.264828718776478Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC699032.2316585651466285Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC506791.6179309103052228Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC409121.3061186961543692Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC376061.2005743959616055Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT351771.1230283871387918No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC337691.078077880583616Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA320321.022624024130249Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC316911.0117375733239176Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA313651.0013299986527617Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA270110.8623282191490435Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG234940.7500477279881391Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC228550.7296476046296468Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT226330.7225602378290438Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG215170.6869318533719584Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA213840.6826858183067324Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT201510.6433221999952751Clontech Universal Primer Mix Long (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT194710.6216131485339685RNA PCR Primer, Index 39 (95% over 23bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA189540.60510788440824Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT188660.6022984777485415Clontech SMART CDS Primer II A (96% over 26bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT135420.432329374836783Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG134340.42888146666351673Clontech Universal Primer Mix Long (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG107050.3417579351371853RNA PCR Primer, Index 39 (96% over 30bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA105240.33597949643939634Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG105050.33537292000150687Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA103250.32962640637939633Clontech SMART CDS Primer II A (96% over 26bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG103180.32940293084964756Clontech SMART CDS Primer II A (96% over 26bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG95120.3036713198528637Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT93660.2990102588038185Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT93590.29878678327406977Clontech SMART CDS Primer II A (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT87420.279089011580502RNA PCR Primer, Index 39 (96% over 25bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC87100.2780674091587934Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG84770.2706288665257281Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA81400.25987011602211Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT68460.21855906809427092Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA67870.21667548862924582Clontech Universal Primer Mix Long (96% over 25bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67280.21479190916422067Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG66560.21249330371537647Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG64030.2044162595687433Clontech SMART CDS Primer II A (96% over 26bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA63250.20192610366582875Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG56340.17986587637205995Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG49610.1583803004405022Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATTAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT46260.1476854000882409Clontech Universal Primer Mix Long (96% over 25bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT44970.14356706532572835Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43480.13881022904964796Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA39320.12552939756743695Clontech Universal Primer Mix Long (96% over 25bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38650.12339041749698468Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTGGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38070.12153876310763793Clontech Universal Primer Mix Long (96% over 25bp)
ACGCTGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37740.12048523561025101Clontech Universal Primer Mix Long (96% over 25bp)
GTGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37720.12042138545889423Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT37280.11901668212904498Clontech SMART CDS Primer II A (96% over 26bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC36680.11710117758834149Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35380.11295091775015054Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT34870.11132273889055255Clontech Universal Primer Mix Long (96% over 28bp)
CTGCATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34250.10934338419849225Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT34120.10892835821467316Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC33780.10784290564160783Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTAGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33610.10730017935507516Clontech Universal Primer Mix Long (96% over 25bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33540.10707670382532641Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCTACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32310.10314991951688422Clontech Universal Primer Mix Long (96% over 25bp)
TGCTAACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32310.10314991951688422Clontech Universal Primer Mix Long (96% over 25bp)
GCATTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG32020.10222409232221086Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)