FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001322631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3593711
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT438241.2194636686144211Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT204260.5683818203522765Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT163990.4563249521177412Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT163240.45423797294774115Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC148290.4126375214924071Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC142810.3973886603569402Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT130750.3638300353033396No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT123390.34334981304840595Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC103530.28808660462680497Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA87650.24389829900067092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC74480.20725094477547024Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC70520.19623169475787008Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT67830.18874639613480326RNA PCR Primer, Index 23 (95% over 23bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA61370.1707705488838696Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC60970.16965749332653626Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA59000.1641756947066695Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC57960.1612817502576028Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA55580.15465906969146936Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55480.154380805802136No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT53630.14923292384946923Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG43290.12046043769240208Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC42010.11689865990893536Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT39320.10941336128586857Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG37750.10504461822333515Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT36380.10123240293946842Clontech Universal Primer Mix Long (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)