FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001322641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2216558
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2122779.576875497956742Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1178735.317839641462123Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT947174.273156849493674Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC786173.5468054524176673Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT748983.37902279119247Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT675063.045532758448008No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT666723.0079068537795988Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC609632.7503453552760635Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC579442.6141431895759104Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC476022.147563925690192Clontech Universal Primer Mix Long (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT453582.0463258800356225RNA PCR Primer, Index 31 (95% over 22bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA376631.699166004228177Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC368451.6622619394574834Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC349121.575054656814755Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC339691.5325112178431604Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG335761.5147810253555287Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA330481.4909603087309242Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA299961.3532693482417333Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT274221.2371433546967867Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC252451.1389280136139004Clontech Universal Primer Mix Long (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT251191.1332435244193926RNA PCR Primer, Index 31 (95% over 24bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT212920.9605884438846175Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG195580.8823590449697234Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG179870.8114833900128037Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG128690.5805848527311264Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT112440.5072729881194176Clontech Universal Primer Mix Long (96% over 25bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT111270.5019945338673746Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT97220.438607967849251Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA91890.41456167625661045Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA91640.41343380141643027Clontech Universal Primer Mix Long (96% over 25bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT88810.4006662582255912RNA PCR Primer, Index 31 (96% over 27bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG86510.39028980969593396Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA85100.383928595597318Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA70390.3175644400011189Clontech Universal Primer Mix Long (96% over 25bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67790.3058345416632454Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC63130.28481095464228773No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG60620.2734870912468792Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG54930.24781665988437931RNA PCR Primer, Index 31 (96% over 29bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG49530.22345456333648836Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT49440.22304852839402353RNA PCR Primer, Index 31 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG48990.22101835368169928RNA PCR Primer, Index 38 (95% over 24bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT47860.21592035940408505Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC46750.21091259511368526Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC46160.20825081049086017Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG44180.1993180417566335Clontech SMART CDS Primer II A (96% over 26bp)
CTTAAACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT44040.1986864318461326No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC43070.1943102774662337Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTGGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40810.18411428891100526Clontech Universal Primer Mix Long (96% over 25bp)
ACGCTGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC40120.1810013543521081Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC40120.1810013543521081Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40010.18050508942242882No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC39960.1802795144543928Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATTAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38820.1751364051831714Clontech Universal Primer Mix Long (96% over 25bp)
GCGTAGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38550.17391830035577685Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38410.17328669044527598Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT37190.16778266122519692Clontech Universal Primer Mix Long (96% over 28bp)
TGCTAACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33300.150232928711994Clontech Universal Primer Mix Long (96% over 25bp)
CTGCATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33120.1494208588270643Clontech Universal Primer Mix Long (96% over 25bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32360.1459921193129167Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32170.14513493443437978Clontech SMART CDS Primer II A (96% over 26bp)
GTGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31520.14220245984991145Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCATTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG30900.1394053302462647Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTTATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG30820.13904441029740708Clontech Universal Primer Mix Long (96% over 25bp)
ATACTCATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT29070.13114928641614612RNA PCR Primer, Index 31 (95% over 22bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA29060.1311041714225389Clontech Universal Primer Mix Long (96% over 25bp)
TGCTGACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27210.1227578976052059Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATA26280.1185622031997358Clontech Universal Primer Mix Long (96% over 25bp)
TATCTACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25790.11635156851298274Clontech Universal Primer Mix Long (96% over 25bp)
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC25660.11576507359608906RNA PCR Primer, Index 38 (96% over 25bp)
CTTAGACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT25170.113554438909336No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24680.11134380422258294Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG24450.11030615936961721Clontech Universal Primer Mix Long (96% over 25bp)
GCGTCGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24160.10899782455500825Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTT24130.10886247957418663Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCTGTCTCT23990.10823086966368577Clontech Universal Primer Mix Long (96% over 25bp)
CTGCCTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23980.10818575467007857Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCT23950.10805040968925694Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23940.10800529469564973Clontech SMART CDS Primer II A (96% over 26bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23770.10723833980432725Clontech Universal Primer Mix Long (96% over 27bp)
AAGCTGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC23750.10714810981711284Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.10696764984268403No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23510.10606534997053992Clontech Universal Primer Mix Long (96% over 25bp)
ACTCAGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23440.10574954501528946Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACAT23240.10484724514314536Clontech Universal Primer Mix Long (96% over 25bp)
ATACTACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC22890.10326822036689318Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22430.10119293066096172Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph