FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001322650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204213
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2647758.263339547027616Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1292474.033658186893319Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1120773.4978011761390393Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC938852.9300486578139466Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT810122.528296339850066Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT761802.377494879397843Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC684672.1367805448639023Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC523711.6344419050793442Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC473081.4764311860665942Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC380861.1886226040528516Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC372591.1628128342279367Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC371981.1609090906253736Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG325771.016692710503328Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA321631.0037722211351119Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA307070.9583320459657332Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC267010.8333091464269073Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG246920.7706104431883898Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT237250.7404314257510347Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA227860.7111262578361676Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG219350.6845674741348343Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT189700.5920330514856533Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG139730.436082120633054Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG108940.33998988207088604Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA97990.30581612395929986Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT90950.28384505025102885Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA74920.23381716508858805Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT73370.22897978380338638Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA71910.22442328272184153Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA64000.19973703371155413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG58710.18322751951883348Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA57800.1803875085707473Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54440.16990131430089073No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA54200.1691523004244724Clontech Universal Primer Mix Long (96% over 25bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT44030.13741283741124577Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC39570.12349366287447182Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT36440.11372527356951613Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT36160.11285142404702808Clontech Universal Primer Mix Long (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)