FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001322663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3533541
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3425079.693024645815628Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1658904.694724074236014Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1382633.912873799964398Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1278893.619287281511662Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1208073.418865098777685Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT1170393.312229856679178No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1078713.052773407751601Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1009722.8575301659157204Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC753812.133299146663361Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT725482.053124613525073RNA PCR Primer, Index 33 (95% over 22bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC624571.7675470583191195Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC549181.5541916734516452Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC532971.5083170111794373Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC495391.401964771315799Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA469941.329940702541728Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA395531.1193587395759663Clontech Universal Primer Mix Long (96% over 25bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT393061.1123685843747109RNA PCR Primer, Index 33 (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA364051.0302696360393158Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC363961.030014934027934Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG341990.9678393430272918Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT321390.9095408826443503Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT315620.8932116536924292Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA303530.8589966834968096Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG301850.8542422459510164Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA237090.6709699986500793Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT210820.5966253115500854Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG206120.5833242065112588Clontech Universal Primer Mix Long (96% over 25bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG197160.5579672062670279RNA PCR Primer, Index 33 (96% over 29bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT184530.5222240240031176Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG162690.4604163359078046Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA154910.4383987620350238Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT150120.42484295498481556Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG135060.382222818413597Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA121780.3446401216230405Clontech Universal Primer Mix Long (96% over 25bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG120100.3398856840772472Clontech SMART CDS Primer II A (96% over 26bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT117060.33128241613724024RNA PCR Primer, Index 33 (96% over 27bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA113550.32134903769335066Clontech SMART CDS Primer II A (96% over 26bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC108610.3073687272908394Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT107560.30439720382471863Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT105350.2981428544341215Clontech SMART CDS Primer II A (96% over 26bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA100510.28444554626647883Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG100390.2841059435846365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT96830.27403106402331256Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA95370.2698992313942303Clontech Universal Primer Mix Long (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC80590.22807150108064403No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG78300.221590749902152Clontech SMART CDS Primer II A (96% over 26bp)
CTTAAACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT78060.2209115445384672No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG77000.2179117208488595Clontech Universal Primer Mix Long (96% over 25bp)
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG76920.21768531906096464RNA PCR Primer, Index 15 (95% over 24bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG75010.21227997637497342Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT68240.1931207250743659Clontech Universal Primer Mix Long (96% over 25bp)
GTATTAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT67310.1904888042900875Clontech Universal Primer Mix Long (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT66720.18881909110436246RNA PCR Primer, Index 33 (95% over 21bp)
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG66680.18870589021041498Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTGGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55950.158339750409009Clontech Universal Primer Mix Long (96% over 25bp)
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC55320.15655683632933648RNA PCR Primer, Index 15 (96% over 25bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC54920.15542482738986188Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCTGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC54750.1549437235905852Clontech Universal Primer Mix Long (96% over 25bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54460.15412301710946613Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTAGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53810.1522835025828199Clontech Universal Primer Mix Long (96% over 25bp)
CTGCATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC51610.14605745341570964Clontech Universal Primer Mix Long (96% over 25bp)
TGCTAACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT51380.14540654827551175Clontech Universal Primer Mix Long (96% over 25bp)
GTGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51300.14518014648761682Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG51050.1444726409004452Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC50380.14257652592682526Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT50370.1425482257033384Clontech Universal Primer Mix Long (96% over 28bp)
CTGCGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49950.14135961631689006Clontech SMART CDS Primer II A (96% over 26bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT47340.1339732579868183Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC47310.13388835731635773Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCTACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC46830.1325299465889882Clontech Universal Primer Mix Long (96% over 25bp)
GCATTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG46820.13250164636550135Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATACTCATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT46690.1321337434601721RNA PCR Primer, Index 33 (95% over 22bp)
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA46140.13057723116839454Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC45400.12848301463036654Clontech Universal Primer Mix Long (96% over 25bp)
CTTAGACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT42930.12149285942911091No Hit
TGCTGACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT42680.1207853538419393Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT41270.11679502233029133Clontech SMART CDS Primer II A (96% over 26bp)
AAGCTGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC38800.10980486712903571Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC38090.10779555126146831Clontech Universal Primer Mix Long (96% over 25bp)
ATACTACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC36770.10405992176120214Clontech Universal Primer Mix Long (96% over 25bp)
CTGCCTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36730.1039467208672547Clontech Universal Primer Mix Long (96% over 25bp)
CTTACACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT35680.10097519740113388No Hit

[FAIL]Adapter Content

Adapter graph