FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001323073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG181.3264554163596167RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG80.5895357406042742RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG70.5158437730287398TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG70.5158437730287398TruSeq Adapter, Index 11 (96% over 28bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA50.36845983787767134RNA PCR Primer, Index 17 (96% over 28bp)
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT40.2947678703021371No Hit
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC40.2947678703021371No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG40.2947678703021371RNA PCR Primer, Index 23 (96% over 30bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA30.2210759027266028RNA PCR Primer, Index 39 (96% over 30bp)
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG30.2210759027266028No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGTCTTCTGCTTG30.2210759027266028Illumina PCR Primer Index 5 (96% over 26bp)
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG30.2210759027266028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2210759027266028No Hit
CCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTC30.2210759027266028No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACCTCTCTA20.14738393515106854No Hit
ATCTAGTACTGGACTGTGTGCCAGAGGAGAGGCGTAAACTGATTGTGGCA20.14738393515106854No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGTCTTCTGCTTG20.14738393515106854Illumina Paired End PCR Primer 2 (96% over 25bp)
GTCTGGAGATGAGATGACATCTCTGTCAGAGTATGTTTCTCGCATGAAGG20.14738393515106854No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA20.14738393515106854RNA PCR Primer, Index 10 (96% over 28bp)
AGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAG20.14738393515106854No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT20.14738393515106854TruSeq Adapter, Index 10 (96% over 26bp)
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTC20.14738393515106854No Hit
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTA20.14738393515106854No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT20.14738393515106854RNA PCR Primer, Index 39 (96% over 28bp)
CCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTA20.14738393515106854No Hit
GGCCAGGCTGGTCTCAAACTCCTAACCTCAGGTGATCTGCTCACCTCGGC20.14738393515106854No Hit
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCG20.14738393515106854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.14738393515106854No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)