FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001323153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG291.627384960718294TruSeq Adapter, Index 3 (96% over 28bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG110.6172839506172839RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG110.6172839506172839TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG90.5050505050505051RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG80.44893378226711567RNA PCR Primer, Index 17 (96% over 28bp)
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT80.44893378226711567No Hit
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG70.3928170594837262No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC60.33670033670033667Illumina PCR Primer Index 12 (95% over 24bp)
CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGG60.33670033670033667No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG60.33670033670033667RNA PCR Primer, Index 46 (96% over 28bp)
GTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTT50.28058361391694725No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA50.28058361391694725RNA PCR Primer, Index 3 (96% over 28bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG40.22446689113355783RNA PCR Primer, Index 33 (96% over 29bp)
ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGG40.22446689113355783No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA40.22446689113355783RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT40.22446689113355783TruSeq Adapter, Index 1 (96% over 26bp)
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTA40.22446689113355783No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT40.22446689113355783RNA PCR Primer, Index 27 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA30.16835016835016833RNA PCR Primer, Index 27 (96% over 30bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG30.16835016835016833TruSeq Adapter, Index 4 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT30.16835016835016833TruSeq Adapter, Index 3 (95% over 21bp)
CTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAA30.16835016835016833No Hit
CTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCA30.16835016835016833No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG30.16835016835016833TruSeq Adapter, Index 3 (95% over 24bp)
CTCCTGACAGATGGAGTTAATATCAGCTCCTGAAATCTTATCTGGCCGGG30.16835016835016833No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG30.16835016835016833No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT30.16835016835016833RNA PCR Primer, Index 3 (95% over 23bp)
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCG30.16835016835016833No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT30.16835016835016833TruSeq Adapter, Index 1 (95% over 21bp)
TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTAC30.16835016835016833No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT30.16835016835016833RNA PCR Primer, Index 33 (96% over 27bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA30.16835016835016833RNA PCR Primer, Index 46 (96% over 28bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT30.16835016835016833TruSeq Adapter, Index 3 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT30.16835016835016833No Hit
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC30.16835016835016833No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACCTCTCTA20.11223344556677892No Hit
CCTCAGAGAGGTTCATCTTGCTAGTGATAGTGGAGAAAATCAATCTCTTC20.11223344556677892No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC20.11223344556677892Illumina PCR Primer Index 5 (95% over 24bp)
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA20.11223344556677892No Hit
GGAAGTGGAAATTCAATTTTACGGTCCAGCCGTCCTGGCCGTAGCAGGGC20.11223344556677892No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC20.11223344556677892No Hit
GGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG20.11223344556677892No Hit
CTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACC20.11223344556677892No Hit
ATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTG20.11223344556677892No Hit
GAGTACATGGGGTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCA20.11223344556677892No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT20.11223344556677892TruSeq Adapter, Index 3 (96% over 27bp)
GGCCAGGAGAGAGCCGGCATCGCTGACAACCTGCAGCACCACGGTGATGG20.11223344556677892No Hit
CCTCTGGGTGTTCTGTACCTCTTTTCTTCTTGCACCCCCGCCTTCCTGCC20.11223344556677892No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG20.11223344556677892RNA PCR Primer, Index 23 (96% over 30bp)
CCGCAGGCGCTGTCACCCAGTGCTACCGAGACATGGGTGCCCGGCACCGC20.11223344556677892No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGTCTTCTGCTTG20.11223344556677892Illumina PCR Primer Index 8 (96% over 26bp)
TCTCCGAGCCCACGAGACCTCTCTACATATCGTATGCCGTCTTCTGCTTG20.11223344556677892TruSeq Adapter, Index 11 (96% over 28bp)
AGCTAACCCCCTACTTCCCAGACAGCTGCTCGTACAGTTTGGGCACATAG20.11223344556677892No Hit
GGCGCGGAGTGCCCAGGTTCACAGTGCAGCTTGTGGCTCGTGTCCATCTT20.11223344556677892No Hit
TACCGGGACCTGACCACCGCAGGCGCTGTCACCCAGTGCTACCGAGACAT20.11223344556677892No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG20.11223344556677892TruSeq Adapter, Index 10 (96% over 28bp)
CTGTCAGAGTATGTTTCTCGCATGAAGGAGACACAGAAGTCCATCTATTA20.11223344556677892No Hit
ACGTAATGCTGAGCACTGCATTGGGAAGGTGGCTGAGGCCCCTGCATCTC20.11223344556677892No Hit
TCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGTCTTCTGCTTG20.11223344556677892TruSeq Adapter, Index 5 (96% over 26bp)
GGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGT20.11223344556677892No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT20.11223344556677892No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGC20.11223344556677892No Hit
AGATTGGGTCTCGTACTCTTCCCAAAGTTCATAGAGCTCCTTTCTGAGGT20.11223344556677892No Hit
GTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATC20.11223344556677892No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCT20.11223344556677892Illumina PCR Primer Index 8 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11223344556677892No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT20.11223344556677892No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT20.11223344556677892No Hit
ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAA20.11223344556677892No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)