FastQCFastQC Report
Wed 31 Aug 2022
EGAF00001323208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG16.25No Hit
TCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGC16.25No Hit
CTCTACTTTGGAGCGACTGCGTAAAAGCGACATTACCCTCACTTTTGCCA16.25No Hit
ATTCAAAATACAGTGATAGAAGTTCAGAAAGCTCACCAAGGTCAAGGAGC16.25No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA16.25No Hit
AAAAGTACTCTGCGTTGATACCGCAGCGTCACCAAGATCTGCTTCTGTGG16.25Clontech SMART CDS Primer II A (95% over 21bp)
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC16.25No Hit
ATGTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGCGTGAAG16.25No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG16.25No Hit
ACTTTATTTTCTGGTAGATAGAAATAAATAGCTATATCCATGTACTGTAG16.25No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCCAGGCAAT16.25No Hit
AGACGGGGTTTTACCGTGTTAGCCAGGAGGTCTCAATCTCCTGACCTCGT16.25No Hit
GGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCACCGTCA16.25No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA16.25No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT16.25No Hit
CACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCAC16.25No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)