FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001323227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74216
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.5807373073191765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.46216449283173444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.322032984801121No Hit
ACTTAATACAGTGAGAAATTTGGACTCTCGGATGAATCAACTGGAGACAA1030.13878408968416514No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.1280047429125795Clontech SMART CDS Primer II A (100% over 21bp)
GTATTAAGTATCTCGTTCACATTCAGAGCAAGTGACTGGACACTGGTGAC880.11857281448744207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)