FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001323282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697886
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA157350.9267406645675859No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA156950.924384793796521No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA133830.7882154632289801No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC106930.6297831538748774No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG104790.617179245249681No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA100570.5923248086149482No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG100570.5923248086149482No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA99840.5880253444577551No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT93990.5535707344309335No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT93350.54980134119723No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG92420.5443239416545045No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC91560.5392588194967153No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA88290.5199995759432612No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA80740.4755325151394146No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC73070.43035869310424846No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT71760.4226432163290115No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT70670.41622346847786007No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG67770.39914340538764087No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG66500.39166351568951036No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT61130.36003595058796645No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG58160.34254361011281087No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC55560.3272304501008902No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA55320.3258169276382513No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT53250.31362529639799136No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC53210.3133897093208849No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC53100.3127418448588421No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA52550.3095025225486281No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT52520.30932583224079824No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA52050.3065576840847972No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT51920.30579202608420114No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA51610.303966226236626No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA51600.30390732946734944No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG51530.30349505208241306No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA50170.295485091460793No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT48990.2885352726861521No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG48540.28588491806870425No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA48280.2843536020675122No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC45630.2687459582092084No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC43380.2554941851219693No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA43070.25366838527439417No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT42730.2516658951189891No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC40010.235645973875749No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC39410.2321121677191519No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA39380.23193547741132206No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC38680.2278127035619588No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT38110.22445558771319157No Hit
ACACAAATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGC38020.223925516789702No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT37520.22098067832587112No Hit
GATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGAT37230.2192726720168492No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT36580.21544438201386903No Hit
GCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACA36000.21202836939582517No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC34460.202958266927226No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT34370.2024281960037364No Hit
CGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCC33910.19971894461701198No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC33360.196479622306798No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT32770.19300471291947752No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA32650.1922979516881581No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT32390.19076663568696603No Hit
AATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTA32090.18899973260866748No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA31850.18758621014602864No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA31170.1835812298352186No Hit
AGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGAT30650.18051859783283447No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA30600.18022411398645138No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC30580.18010632044789815No Hit
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG30510.17969404306296183No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC30440.17928176567802548No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT30390.1789872818316424No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT30060.177043688445514No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA29870.17592464982925826No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA29560.17409884998168312No Hit
GTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGA29330.17274422428832087No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG29250.17227305013410793No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG28950.17050614705580938No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG28780.1695049019781069No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT28600.16844476013112777No Hit
GCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCA28380.16714903120704216No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC27840.16396860566610477No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT27780.1636152250504451No Hit
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC27540.16220170258780625No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC27540.16220170258780625No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT26780.15772554812278328No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG26730.15743106427640016No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC26620.1567831998143574No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG26240.15454512258184588No Hit
GAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTA26040.15336718719631354No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA25740.151600284118015No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC25520.15030455519392938No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT25500.15018676165537617No Hit
ATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAGCG25040.14747751026865172No Hit
CGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGC25000.14724192319154525No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG24840.14629957488311937No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG24330.14329583965001183No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG24160.14229459457230934No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT23880.14064548503256402No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT23820.14029210441690432No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC23770.13999762057052123No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC23570.13881968518498886No Hit
CTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACG23080.13593374349043458No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC22920.13499139518200867No Hit
TATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCG22700.1336956662579231No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG22660.13346007918081662No Hit
CTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGA22510.13257662764166736No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA22350.13163427933324146No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG22320.1314575890254116No Hit
ACGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTG22190.13069193102481558No Hit
CCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATC21980.12945509887000659No Hit
GCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCACCTTCC21920.12910171825434688No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA21880.1288661311772404No Hit
CATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAG21820.12851275056158068No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC21770.1282182667151976No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC21750.12810047317664439No Hit
GGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCT21630.12739371194532495No Hit
ACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGC21200.12486115086643038No Hit
GATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCG21100.12427218317366417No Hit
GAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCC21000.123683215480898No Hit
GGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAAT20980.12356542194234478No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG20810.12256417686464227No Hit
TTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGC20770.1223285897875358No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC20740.12215189947970594No Hit
CACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG20590.12126844794055668No Hit
GTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACT20540.12097396409417357No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA20400.12014940932430092No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG20380.1200316157857477No Hit
GTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAAT20360.11991382224719445No Hit
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG20210.11903037070804519No Hit
AAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTAT20140.11861809332310885No Hit
CTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCC19840.11685119024481032No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT19760.11638001609059737No Hit
CACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCAC19730.1162033257827675No Hit
GAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAG19680.11590884193638441No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT19420.11437752593519235No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC19200.11308179701110675No Hit
GCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGG19140.11272841639544705No Hit
TCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGG19110.1125517260876172No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG19050.11219834547195748No Hit
CTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGG19030.11208055193340424No Hit
AATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCG18930.11149158424063807No Hit
TCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAG18770.11054923593221218No Hit
CAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCG18720.11025475208582909No Hit
ATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGAC18670.109960268239446No Hit
GCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGC18360.10813446839187082No Hit
ACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAA18340.1080166748533176No Hit
TGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAG18340.1080166748533176No Hit
AGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCC18320.10789888131476436No Hit
GACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGT17960.10577859762080612No Hit
TGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGA17790.10477735254310361No Hit
CACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGC17670.10407059131178419No Hit
CTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTC17660.10401169454250757No Hit
TTCTTATACAGGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCG17640.10389390100395432No Hit
CTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGT17440.10271596561842197No Hit
GCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACCGCTAT17370.10230368823348564No Hit
ATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCA17300.1018914108485493No Hit
TACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAG17130.10089016577084679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)