FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001323386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627501
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA145490.8939472233811224No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA131030.8050993517054674No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC112650.6921654733238259No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA109590.6733636415584384No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA102710.6310902420336454No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG90240.5544697053949583No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT88800.5456217845641878No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG81350.4998460830438814No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA79100.4860212067458023No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA77870.47846360770285246No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA75460.4636556290902432No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG74720.45910878088554175No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC73620.45234995247314747No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG72630.4462670069019927No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT67530.4149306206263467No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC65970.40534537305967866No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG65780.40417793906117416No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC60420.3712440115244169No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT58550.3597540032233467No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT57060.3505988629192855No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA56070.3445159173481307No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA55620.3417509420885148No Hit
TTCTTATACAGGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCG53780.33044526547141906No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA52140.32036846674748587No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG50520.310414555812869No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC48150.2958523527788923No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC46330.28466956395111276No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT46190.28380934942589897No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT45740.28104437416628314No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC44120.27109046323166625No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC43500.2672809417628622No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC43210.26549906881777646No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT42180.2591703476679892No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA41660.2559752651457664No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT41600.25560660177781763No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT40340.24786467105089335No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT39540.242949159478243No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA39170.24067573537589226No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA39030.23981552085067842No Hit
GTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGA39010.23969263306136218No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT38730.23797220401093455No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC37520.2305374927573009No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG37090.22789540528700136No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA37020.22746529802439444No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC36930.22691230297247128No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT36160.22218112308379534No Hit
GATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGAT35550.21843304550964948No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA35260.21665117256456373No Hit
TACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCG34970.21486929961947795No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT34450.21167421709725526No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC34350.21105977815067395No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA34120.20964656857353695No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC33970.20872491015366504No Hit
GTGTAAGTAATGCCGTCATCGGAAACGGTATAACTCTCCGCCAGCACGTT33710.20712736889255368No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC33390.20516116426349354No Hit
AGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGAT33370.20503827647417727No Hit
GCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACA33320.20473105700088662No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT32660.2006757599534501No Hit
CGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCC32140.1974806774312274No Hit
AATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTA32080.1971120140632786No Hit
GATATAACGCTGCATAATTGACGGACTGGCCATCAACTCGATATTTTTGT32000.19662046290601357No Hit
ACACAAATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGC31770.1952072533288766No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG31630.19434703880366277No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA31110.19115195628144008No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC30940.19010741007225188No Hit
GTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGA30810.1893086394416962No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT30630.18820264933784986No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA30510.1874653226019523No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG29840.18334858165985768No Hit
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG29410.1807064941895581No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA29100.1788017334551561No Hit
CCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATC28850.17726563608870285No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC28710.17640542156348904No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT28600.17572953872224964No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT28580.17560665093293337No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG28310.1739476657771639No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG28230.17345611461989885No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA28110.1727187878840013No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC28110.1727187878840013No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC27820.17093691493891555No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG27490.1689092664151973No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG27120.1666358423128465No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA26860.16503830105173514No Hit
ATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAGCG26590.16337931589596566No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT26500.1628263208440425No Hit
GGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCGGTCCAGATTC26270.16141311126690552No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC26120.1604914528470336No Hit
GCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCA25980.15963123832181977No Hit
TATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCG25120.15434706338122067No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT24980.15348684885600686No Hit
GGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAAT24970.1534254049613487No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC24940.15324107327737432No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG24910.15305674159339996No Hit
GAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTA24860.1527495221201093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.15250374654147678No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT24540.15078331749104915No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG24280.1491857762299378No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT23690.14556058644510816No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC23310.14322571844809928No Hit
GAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAG23290.143102830658783No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT23090.14187395276562043No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA22790.14003063592587653No Hit
GATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCG22490.13818731908613266No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA22230.1365897778250213No Hit
GGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCT22010.13523801214254247No Hit
ACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA21940.13480790487993555No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT21900.13456212930130304No Hit
GACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGT21880.13443924151198677No Hit
CACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGC21780.1338248025654055No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG21560.13247303688292664No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG21500.1321043735149779No Hit
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC21460.13185859793634536No Hit
ACGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTG21200.130261056675234No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA21110.12970806162331083No Hit
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG21020.12915506657138767No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG20960.1287864032034389No Hit
GTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAAT20910.12847918373014824No Hit
GCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCACCTTCC20840.12804907646754135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20820.12792618867822508No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC20810.12786474478356696No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC20760.1275575253102763No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG20620.1266973107850625No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA20580.12645153520642996No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT20530.12614431573313933No Hit
CTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGA20490.12589854015450683No Hit
TACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAG20430.12552987678655803No Hit
CTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGG20120.12362511605215604No Hit
CAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCG19890.12221190647501907No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT19860.12202757479104467No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG19730.121228804160489No Hit
CGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGC19700.1210444724765146No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC19600.12043003352993331No Hit
AATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCG19480.11969270679403578No Hit
TCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAG19440.11944693121540326No Hit
CTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGTAATGTCGCGTTCAC19340.11883249226882196No Hit
ACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGC19140.11760361437565937No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC19100.11735783879702685No Hit
CTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCC18960.11649762427181304No Hit
CATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT18860.11588318532523174No Hit
GTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATC18820.11563740974659922No Hit
ATATAACGCTGCATAATTGACGGACTGGCCATCAACTCGATATTTTTGTT18780.11539163416796673No Hit
GTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGGGTATCCCA18740.11514585858933421No Hit
CACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCAC18540.11391698069617162No Hit
CCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTG17990.11053756648997451No Hit
AGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCC17850.1096773519647607No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG17660.10850991796625624No Hit
ATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCAGGGAT17600.10814125459830747No Hit
TGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAG17490.10746537175706805No Hit
TTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATG17420.10703526449446114No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG17390.10685093281048676No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC17320.10642082554787986No Hit
GCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACCGCTAT17060.10482328428676847No Hit
TGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGA16960.10420884534018719No Hit
TCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAG16960.10420884534018719No Hit
GAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCC16880.10371729418292217No Hit
ATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCA16870.10365585028826405No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG16770.10304141134168275No Hit
GCGCATGACGCCCCGTGGCAAAAGTGAGGGTAATGTCGCGTTCACGCAGT16760.1029799674470246No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT16760.1029799674470246No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA16670.10242697239510146No Hit
GTACATGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTG16490.10132098229125512No Hit
CTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTC16390.10070654334467383No Hit
CACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG16370.10058365555535757No Hit
CTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACA16320.10027643608206693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)