FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001323409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG90.6122448979591837No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG90.6122448979591837RNA PCR Primer, Index 31 (96% over 29bp)
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT80.5442176870748299No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT70.4761904761904762No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG70.4761904761904762No Hit
CTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCG50.3401360544217687No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA50.3401360544217687No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG50.3401360544217687No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT50.3401360544217687No Hit
GAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGG50.3401360544217687No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA40.27210884353741494No Hit
GATATAACGCTGCATAATTGACGGACTGGCCATCAACTCGATATTTTTGT40.27210884353741494No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT40.27210884353741494No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA40.27210884353741494No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT40.27210884353741494No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT40.27210884353741494No Hit
AGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTC40.27210884353741494No Hit
GGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCGGTCCAGATTC40.27210884353741494No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT40.27210884353741494No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG40.27210884353741494No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT40.27210884353741494No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC40.27210884353741494No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA40.27210884353741494No Hit
ATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGAC30.20408163265306123No Hit
TCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG30.20408163265306123No Hit
CCTGGAATCAGACCGATTTTGTGAAGGTGAAAAAATTTGCGGGTTACTGG30.20408163265306123No Hit
CCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATC30.20408163265306123No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC30.20408163265306123No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG30.20408163265306123No Hit
ACGCAGAGTACATGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGG30.20408163265306123No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA30.20408163265306123RNA PCR Primer, Index 31 (96% over 29bp)
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG30.20408163265306123No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA30.20408163265306123No Hit
ACATGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTG30.20408163265306123No Hit
GATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGG30.20408163265306123No Hit
GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTT30.20408163265306123No Hit
TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATA30.20408163265306123No Hit
ACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA30.20408163265306123No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC30.20408163265306123No Hit
CATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTC30.20408163265306123No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA30.20408163265306123No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT30.20408163265306123No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA30.20408163265306123No Hit
TTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATC30.20408163265306123No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG30.20408163265306123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.20408163265306123No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA20.13605442176870747No Hit
TATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCG20.13605442176870747No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC20.13605442176870747No Hit
CGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGGGTATCC20.13605442176870747No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20.13605442176870747Clontech Universal Primer Mix Long (96% over 25bp)
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG20.13605442176870747No Hit
GTACATGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTG20.13605442176870747No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG20.13605442176870747No Hit
GGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGC20.13605442176870747No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG20.13605442176870747No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC20.13605442176870747No Hit
AGTGTGGCCTGCTCGTAAGGAATGGGGAAAGCAAACTGCGCTTCACCGGT20.13605442176870747No Hit
GCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGA20.13605442176870747No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA20.13605442176870747No Hit
GAGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGG20.13605442176870747No Hit
ATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTG20.13605442176870747No Hit
TGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCAC20.13605442176870747No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC20.13605442176870747No Hit
CATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG20.13605442176870747No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA20.13605442176870747No Hit
ATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAAC20.13605442176870747No Hit
AGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTC20.13605442176870747No Hit
ATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGC20.13605442176870747No Hit
ATTCAGCGCCTCACGGACCTTCGGGTTATCGAACGGCTTTTGCGTCACGT20.13605442176870747No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC20.13605442176870747No Hit
ACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAA20.13605442176870747No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA20.13605442176870747No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA20.13605442176870747No Hit
CGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCT20.13605442176870747No Hit
ACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCG20.13605442176870747No Hit
GATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCG20.13605442176870747No Hit
GTTTAAGATGATTCGCCGGATCGCTGGCCCGGTCCAGATTCGCTTTCACC20.13605442176870747No Hit
CAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGG20.13605442176870747No Hit
TATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGT20.13605442176870747No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG20.13605442176870747No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA20.13605442176870747No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT20.13605442176870747No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC20.13605442176870747No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG20.13605442176870747TruSeq Adapter, Index 1 (96% over 28bp)
AGTCAGCGTCGGTAGCGGATTTATTATGGGCAATGCGATGCCGCAACTGC20.13605442176870747No Hit
GTATCAACGCAGAGTACATGGGGGAATTCGTGGAGAAAGAAATGGCTCGT20.13605442176870747No Hit
TACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCG20.13605442176870747No Hit
AGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAAC20.13605442176870747No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT20.13605442176870747No Hit
GGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCT20.13605442176870747No Hit
CCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTG20.13605442176870747No Hit
AGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACC20.13605442176870747No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC20.13605442176870747No Hit
GAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCC20.13605442176870747No Hit
TCTTTACCGTTGTCGGATCGATCGCTTCCGTTTTAGCAATATTCTTATAC20.13605442176870747No Hit
CCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCACCGTCAAT20.13605442176870747No Hit
AGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCG20.13605442176870747No Hit
TTGCAGCATTGCCGCGCGGGTGTTGTTATCCGCCACCGGACGCCAGGTTA20.13605442176870747No Hit
GAACAGCCCCTGGTAAAACGATTTCGCTACGGCCTGAGATAACGTGTCGT20.13605442176870747No Hit
TTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAA20.13605442176870747No Hit
GTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTC20.13605442176870747No Hit
CCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCG20.13605442176870747No Hit
TAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTC20.13605442176870747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13605442176870747No Hit
CAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCG20.13605442176870747No Hit
GATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCT20.13605442176870747No Hit
TTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCG20.13605442176870747No Hit
CATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAG20.13605442176870747No Hit
GGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA20.13605442176870747No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA20.13605442176870747No Hit
CGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGC20.13605442176870747No Hit
GAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGG20.13605442176870747No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC20.13605442176870747No Hit
AGTGAGGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTT20.13605442176870747No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA20.13605442176870747No Hit
CTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGG20.13605442176870747No Hit
GGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAG20.13605442176870747No Hit
TTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGT20.13605442176870747No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCATCTGCGTGCGAAGAGGTGG20.13605442176870747No Hit
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA20.13605442176870747No Hit
CTCCAGCAGTGTGGCCTGCTCGTAAGGAATGGGGAAAGCAAACTGCGCTT20.13605442176870747No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20.13605442176870747No Hit
GTACATGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATT20.13605442176870747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13605442176870747No Hit
CTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTC20.13605442176870747No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC20.13605442176870747No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG20.13605442176870747No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA20.13605442176870747No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA20.13605442176870747No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT20.13605442176870747No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA20.13605442176870747No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA20.13605442176870747No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC20.13605442176870747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)