FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001323410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA90.6122448979591837No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA90.6122448979591837No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA70.4761904761904762No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC60.40816326530612246No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT60.40816326530612246No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT50.3401360544217687No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG50.3401360544217687No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC50.3401360544217687No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT40.27210884353741494No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA40.27210884353741494No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG40.27210884353741494No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT40.27210884353741494No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA40.27210884353741494No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA40.27210884353741494No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT30.20408163265306123No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG30.20408163265306123No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA30.20408163265306123No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG30.20408163265306123No Hit
GGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCT30.20408163265306123No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA30.20408163265306123No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG30.20408163265306123No Hit
ATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGTAATGT30.20408163265306123No Hit
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG30.20408163265306123No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC30.20408163265306123No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.20408163265306123No Hit
AGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGAT30.20408163265306123No Hit
TATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCG20.13605442176870747No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA20.13605442176870747No Hit
GACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGT20.13605442176870747No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC20.13605442176870747No Hit
TTCTTATACAGGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCG20.13605442176870747No Hit
CGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGC20.13605442176870747No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC20.13605442176870747No Hit
GTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTAT20.13605442176870747No Hit
GGGCTGTTCGGTCTGGATAAAGAGATGAAACCGAAAAACGTGCTGGCGGA20.13605442176870747No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG20.13605442176870747No Hit
GGCAAGAATATTAATAAACGCTGAGAACGGCTGTTTGAGGGTAATCTTTA20.13605442176870747No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG20.13605442176870747No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC20.13605442176870747No Hit
ATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTG20.13605442176870747No Hit
GAGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGG20.13605442176870747No Hit
TAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGA20.13605442176870747No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC20.13605442176870747No Hit
ATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAGCG20.13605442176870747No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA20.13605442176870747No Hit
TGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAG20.13605442176870747No Hit
CGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCC20.13605442176870747No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG20.13605442176870747No Hit
GATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCG20.13605442176870747No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC20.13605442176870747No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA20.13605442176870747No Hit
TACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAG20.13605442176870747No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA20.13605442176870747No Hit
GCTCGTAAGGAATGGGGAAAGCAAACTGCGCTTCACCGGTTTGCAGCATT20.13605442176870747No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA20.13605442176870747No Hit
GGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG20.13605442176870747No Hit
CGCCTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTG20.13605442176870747No Hit
AGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATA20.13605442176870747No Hit
ATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCATCACCA20.13605442176870747No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT20.13605442176870747No Hit
TGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGA20.13605442176870747No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC20.13605442176870747No Hit
GCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTG20.13605442176870747No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG20.13605442176870747No Hit
TATCAACGCAGAGTACATGGGGGGGAATTCGTGGAGAAAGAAATGGCTCG20.13605442176870747No Hit
AGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAG20.13605442176870747No Hit
GAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGG20.13605442176870747No Hit
GCGTTATATCAGTATGAACGTGACGCAAAAGCCGTTCGATAACCCGAAGG20.13605442176870747No Hit
CATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTC20.13605442176870747No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA20.13605442176870747No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC20.13605442176870747No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG20.13605442176870747No Hit
TTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATC20.13605442176870747No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG20.13605442176870747No Hit
TTGACGGACTGGCCATCAACTCGATATTTTTGTTTTTCTCCAGCAGTGTG20.13605442176870747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)