FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001323547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200462
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG174460.7928335049639575No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT160490.7293468371641955No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT119800.5444311240094125No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA117350.533297098518402No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA111100.5048939722658242No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG109470.4974864369391519No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG104300.4739913709030195No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG98820.4490875098047592No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC97620.4436341095642642No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG93590.42531977375660207No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT92290.4194119234960658No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT86000.3908270172354715No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC75240.34192819507903344No Hit
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA74790.3398831699888478No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA74540.3387470449387447No Hit
GTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGA73840.33556589479845594No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT71580.3252953243455238No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA69860.31747878400081436No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC69330.31507019889459575No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC67180.305299523463709No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG67070.3047996284416636No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT66210.3008913582693089TruSeq Adapter, Index 10 (95% over 21bp)
GCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGT64670.2938928279606737No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC63070.28662162764001375No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC62860.2856672825979272No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG62300.28312236248569617No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA60970.2770781772191476No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG60200.27357891206483004No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT58820.2673075017882608No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT58230.2646262466700175No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC56740.2578549413714029No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA56140.2551282412511554No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC54970.24981117601667285No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG52810.23999505558378195No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG52700.2394951605617366No Hit
GTGTAAGTAATGCCGTCATCGGAAACGGTATAACTCTCCGCCAGCACGTT51940.23604134040942312No Hit
GTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCA51220.23276930026512616No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA50690.23036071515890755No Hit
GCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCT49980.22713412001661468No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA49950.2269977850106023No Hit
TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATA49500.22495275992041672No Hit
ACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAA49420.2245891999043837No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG48140.2187722396478558No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT47970.21799967461378564No Hit
TTCTTATACAGGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCG47850.21745433458973618No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA47750.21699988456969488No Hit
AGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCC47610.2163636545416372No Hit
GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTT47320.21504574948351757No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC47180.2144095194554598No Hit
TGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCG46770.2125462743732907No Hit
AAAGTAGAGAGGGTTTTCTCACCTAAATGATGGTCGGGCACCAATAAAGT46250.21018313426907623No Hit
CATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTT44300.20132135887827193No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT44110.20045790384019355No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT44070.20027612383217708No Hit
ACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA43770.19891277377205333No Hit
GTAGAGAGGGTTTTCTCACCTAAATGATGGTCGGGCACCAATAAAGTGCC43730.19873099376403683No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC43500.19768575871794195No Hit
CCAATAAAGTGCCATCCATATCAAATGCTGCCAGACGAGCCATTTCTTTC42230.19191424346341812No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT41820.19005099838124903No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC40920.1859609482008778No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG40540.18423403812472108No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA40030.18191634302251072No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC39820.18096199798042412No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG39240.1783261878641849No Hit
CCTAAATGATGGTCGGGCACCAATAAAGTGCCATCCATATCAAATGCTGC38900.17678105779604464No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT38800.1763266077760034No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA38260.17387257766778066No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT38170.17346357264974355No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA37510.17046420251747132No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA37110.16864640243730633No Hit
CATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG36510.16591970231705888No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG35990.1635565622128444No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA35940.16332933720282378No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT35600.16178420713468353No Hit
CTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGC35540.1615115371226588No Hit
AAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA34750.15792138196433295No Hit
GCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGA34750.15792138196433295No Hit
TACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCG34700.15769415695431233No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG34330.1560126918801597No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC33830.15374044177995347No Hit
CATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT33480.15214986670980912No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC33080.15033206662964416No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA32980.1498776166096029No Hit
AGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGA32930.14965039159958227No Hit
GTACATGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTG32660.14842337654547091No Hit
ATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGC32490.14765081151140078No Hit
GGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATG32380.1471509164893554No Hit
TCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG32020.14551489641720694No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA31990.14537856141119454No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG31940.14515133640117395TruSeq Adapter, Index 10 (96% over 28bp)
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT31580.14351531632902545No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA31240.14197018626088523No Hit
GATATAACGCTGCATAATTGACGGACTGGCCATCAACTCGATATTTTTGT30790.1399251611706996No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT30140.13697123604043152No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT29710.13501710095425415No Hit
GAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCC29570.1343808709261964No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG29400.1336083058921263No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC29190.1326539608500397No Hit
CTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCG29070.13210862082599018No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA28980.13169961580795306No Hit
CGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGC28910.1313815007939242No Hit
TCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATA28840.1310633857798953No Hit
TTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATC28770.13074527076586645No Hit
GATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCT27800.12633710557146635No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC27780.1262462155674581No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT27720.12597354555543336No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT27410.1245647504933055No Hit
TCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCAT27070.12301962042516525No Hit
GTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGG26960.1225197254031199No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT26760.12161082536303738RNA PCR Primer, Index 10 (95% over 23bp)
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT26370.11983847028487653No Hit
GATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATAC26060.11842967522274868No Hit
GGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCGGTCCAGATTC25900.11770255519068268No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC25850.11747533018066207No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG25740.11697543515861669No Hit
GCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACCGCTAT25630.11647554013657133No Hit
GCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAG25320.11506674507444346No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC25200.11452140505039396No Hit
CGCCAAAGTAGAGAGGGTTTTCTCACCTAAATGATGGTCGGGCACCAATA25160.11433962504237746No Hit
ATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCAGGGAT25020.11370339501431971No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT24920.11324894499427848No Hit
GTACATGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATT24440.1110675848980805No Hit
CTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGA24390.11084035988805988No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA24340.11061313487803925No Hit
GCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTT24270.11029501986401039No Hit
CATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAG24260.11024957486200625No Hit
CGTTCATACTGATATAACGCTGCATAATTGACGGACTGGCCATCAACTCG24220.11006779485398975No Hit
CACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG24120.10961334483394851No Hit
TTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCG23910.10865899979186189No Hit
GGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCT23870.10847721978384539No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG23660.10752287474175878No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA23420.10643219469365979No Hit
GGATAAAGAGATGAAACCGAAAAACGTGCTGGCGGAGAGTTATACCGTTT23330.10602318967562266No Hit
TTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGC23240.10561418465758554No Hit
AGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATC23130.10511428963554018No Hit
AGTGAGGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTT23120.10506884463353605No Hit
TGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTAC23100.10497795462952779No Hit
CCAAAGTAGAGAGGGTTTTCTCACCTAAATGATGGTCGGGCACCAATAAA23010.10456894961149069No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG22740.10334193455737932No Hit
ATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGG22740.10334193455737932No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA22600.10270570452932157No Hit
CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTG22550.10247847951930095No Hit
ACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAA22540.10243303451729682No Hit
GATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCAG22430.10193313949525146No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT22430.10193313949525146No Hit
TGGCAAAAGTGAGGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAG22330.1014786894752102No Hit
TTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCC22290.1012969094671937No Hit
ATAAAGTGCCATCCATATCAAATGCTGCCAGACGAGCCATTTCTTTCTCC22210.10093334945116071No Hit
GTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACT22030.10011533941508646No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)