FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001323574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103880
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA171800.8165864973287449No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG163850.7787991710553834No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC163350.776422609654543No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT147930.7031294560526266No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA135780.645379014012206No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC129450.6152917466775671No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA120640.5734167347947602No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG115800.5504116204346255No Hit
CCAATAAAGTGCCATCCATATCAAATGCTGCCAGACGAGCCATTTCTTTC113590.5399072190429112No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT108670.5165218548586421No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA105750.5026427362777345No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC104420.4963210829514991No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG94660.4499306044070955No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG89600.4258798030305911No Hit
GATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGAT84240.4004030648135825No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC81460.38718938342491016No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG81430.3870467897408597No Hit
CCTAAATGATGGTCGGGCACCAATAAAGTGCCATCCATATCAAATGCTGC78680.3739757020362378No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA78600.37359545221210333No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA74610.3546304922333973No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA73800.35078046276403596No Hit
GTAGAGAGGGTTTTCTCACCTAAATGATGGTCGGGCACCAATAAAGTGCC72480.3445063406658175No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT69520.3304370971728426No Hit
AAAGTAGAGAGGGTTTTCTCACCTAAATGATGGTCGGGCACCAATAAAGT69010.3280130045439854No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC68000.32321235051428787No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC67740.3219765385858509No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA66180.314561667015229No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC64180.3050554214118676No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC62330.2962621442287583No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA61020.2900355533585566No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC60110.28571021160902715No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT60060.28547255546894307No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG58560.27834287126642204No Hit
ATAAAGTGCCATCCATATCAAATGCTGCCAGACGAGCCATTTCTTTCTCC57410.27287678004448923No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA57050.27116565583588415No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA56930.2705952810996825No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT56880.2703576249595985No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT55240.2625625035648421No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC52820.2510599463847748No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT52640.25020438428047226No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT52250.2483506663878168No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC51350.24407285586630417No Hit
GAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTA51310.24388273095423693No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG51090.2428370439378672No Hit
CGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCC50950.24217160674563187No Hit
GTGTAAGTAATGCCGTCATCGGAAACGGTATAACTCTCCGCCAGCACGTT49490.23523204745517803No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA49250.23409129798277467No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT48990.2328554860543377No Hit
GCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACA48390.23000361237332928No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA46060.21892883624541323No Hit
AAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTAT45460.2160769625644048No Hit
AGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGAT45440.2159819001083712No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG44960.21370040116356448No Hit
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA42140.20029659486282486No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG41620.19782497100595092No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA41550.19749225240983326No Hit
ACACAAATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGC41380.19668422153354756No Hit
GTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGA40810.19397494153658956No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA40240.19126566153963154No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC40000.19012491206722817No Hit
CGCCAAAGTAGAGAGGGTTTTCTCACCTAAATGATGGTCGGGCACCAATA39970.18998231838317775No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG39900.1896495997870601No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG39370.18713044470216933No Hit
TGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGA38020.1807137289199004No Hit
AATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTA37980.18052360400783315No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT37140.17653098085442137No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT36850.17515257524193395No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC36460.17329885734927847No Hit
GGGTTTTCTCACCTAAATGATGGTCGGGCACCAATAAAGTGCCATCCATA36270.17239576401695916No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC36150.17182538928075744No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG36110.17163526436869023No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA35990.17106488963248853No Hit
CCAAAGTAGAGAGGGTTTTCTCACCTAAATGATGGTCGGGCACCAATAAA35950.17087476472042132No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC35620.16930623419586668No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA35550.16897351559974902No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT35270.16764264121527844No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC35150.16707226647907675No Hit
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC35130.16697720402304314No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA34710.16498089244633724No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT34200.16255679981748009No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA33780.1605604882407742No Hit
ATCCATATCAAATGCTGCCAGACGAGCCATTTCTTTCTCCACGAATTCCC33380.1586592391201019No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG32740.15561724052702627No Hit
GCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACCGCTAT32660.1552369907028918No Hit
ACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA32540.15466661596669012No Hit
GTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGA32160.15286042930205146No Hit
AGTGAGGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTT31780.15105424263741277No Hit
GCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGT31330.14891533737665646No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30900.14687149457193377Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGGCAAAAGTGAGGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAG30590.14539802650341274No Hit
ATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCAGGGAT30500.14497024545126147No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC30280.14392455843489171No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT30120.14316405878662283No Hit
CTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGA29800.141643059490085No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC28890.13731771774055554No Hit
GTACATGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTG28890.13731771774055554No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT28810.13693746791642109No Hit
CACCTAAATGATGGTCGGGCACCAATAAAGTGCCATCCATATCAAATGCT28800.1368899366884043No Hit
TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATA28650.13617696826815218No Hit
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG28210.13408559423541266No Hit
GGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCT28130.1337053444112782No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG28120.1336578131832614No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA28110.1336102819552446No Hit
CTGCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACCGCT27630.13132878301043785No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC27550.1309485331863034No Hit
GATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCAG27410.1302830959940681No Hit
GCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGC27410.1302830959940681No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT27270.1296176588018328No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG27230.12942753388976558No Hit
GTGCCATCCATATCAAATGCTGCCAGACGAGCCATTTCTTTCTCCACGAA27200.12928494020571515No Hit
TGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCGA27040.12852444055744625No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA26960.1281441907333118No Hit
GACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGT26470.12581516056048822No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT26280.12491206722816892No Hit
TATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCG26190.12448428617601763No Hit
CATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT25940.12329600547559746No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC25890.12305834933551342No Hit
ACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGC25790.12258303705534536No Hit
GGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAAT25660.12196513109112687No Hit
AGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGG25610.12172747495104283No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA25480.12110956898682435No Hit
GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTT25430.12087191284674031No Hit
ACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACC25330.12039660056657224No Hit
GATATAACGCTGCATAATTGACGGACTGGCCATCAACTCGATATTTTTGT25140.1194935072342529No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA25140.1194935072342529No Hit
CTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGT25100.11930338232218568No Hit
GCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCA24580.1168317584653117No Hit
GTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTT24400.1159761963610092No Hit
CGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGC24360.11578607144894196No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT24100.11455025952050499No Hit
CTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGG24090.11450272829248817No Hit
TTTCTCACCTAAATGATGGTCGGGCACCAATAAAGTGCCATCCATATCAA24030.11421754092438732No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA23960.11388482232826969No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG23670.11250641671578228No Hit
TGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCG23440.1114131984713957No Hit
TTCTTATACAGGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCG23180.11017738654295872No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG23180.11017738654295872No Hit
AAATGATGGTCGGGCACCAATAAAGTGCCATCCATATCAAATGCTGCCAG23120.10989219917485787No Hit
GCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAG23020.10941688689468981No Hit
AACGAAAACCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTC22690.10784835637013518No Hit
AGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCC22480.10685020058178224No Hit
CAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGAT22250.10575698233739567No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG21840.10380820198870658No Hit
CTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTC21780.10352301462060573No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT21780.10352301462060573No Hit
CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTG21650.10290510865638725No Hit
TCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCT21490.10214460900811832No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC21250.10100385953571496No Hit
GTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCACC21170.10062360971158052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)