FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001323685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621663
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT96330.5940198425936831No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA91630.5650372487995348No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG79920.49282742468688007No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG77520.4780278023239107No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA73390.4525601188409676No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG71390.44022710020515976No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT67200.41438942616314245No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT66430.4096412139883564No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA63150.3894150634256316No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG63040.38873674740066216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61410.37868533721247877No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT59740.3683872666515793No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA58950.3635157242904352No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC57720.3559309178294134No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG57330.3535259791954309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54070.3334231588190641No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA51650.3185002062697367No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA50040.3085721262679114No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA48610.2997540179433088No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG47700.2941424944640163No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG46650.2876676596802172No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT44520.27453299483308186No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC43080.26565322141530023No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG42170.2600416979360076No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC42040.2592400517246802No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC39810.24548873594575446No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT37660.23223074091226104No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC37370.23044245321006893No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA36390.22439927407852311No Hit
GTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCA34730.21416286861080264No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC34640.21360788277219128No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT34010.2097229819019118No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG33870.20885967059740526No Hit
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA33150.20441978388851442No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG32630.2012131990432044No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT31010.1912234539482001No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC30740.18955849643236602No Hit
GCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGT30630.18888018040739663No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC30630.18888018040739663No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG29730.1833303220212831No Hit
ACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAA29630.18271367108949269No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG28650.17667049195794687No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT28470.17556052028072416No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC28340.17475887406939666No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC27710.1708739731991172No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT27250.16803737891288142No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT26810.1653241148130037No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT26580.1639058176698858No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT26370.16261085071312598No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC26280.16205586487451462No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT26090.16088422810411288No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG25970.1601442469859644No Hit
GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTT25900.15971259133371113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.15928093568145787No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG25720.15860261965648842No Hit
GATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCT25630.15804763381787706No Hit
TTCTTATACAGGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCG25560.1576159781656238No Hit
GCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGA25490.15718432251337053No Hit
GTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGA25300.1560126857429688No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA25030.15434772822713472No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG24710.1523744452454055No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT24630.15188112449997318No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA24060.14836621418876794No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG23850.14707124723200815No Hit
GATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATAC23810.14682458685929198No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT23500.14491296897074177No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC23450.14460464350484656No Hit
TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATA23070.14226136996404307No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT23000.1418297143117898No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA22930.14139805865953653No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC22880.14108973319364135No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA22880.14108973319364135No Hit
TGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCG22640.1396097709573444No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT22620.13948644077098632No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG22530.13893145493237496No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC22230.1370815021370038No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA22220.13701983704382475No Hit
GCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCT21640.13344326163944048No Hit
CTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCG21580.13307327108036626No Hit
GTGTAAGTAATGCCGTCATCGGAAACGGTATAACTCTCCGCCAGCACGTT21570.13301160598718723No Hit
ACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA21510.13264161542811298No Hit
GAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCC21280.1312233182849951No Hit
TACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCG21240.13097665791227894No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA21180.1306066673532047No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT21110.13017501170095142No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG21100.13011334660777238No Hit
GTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACT21080.1299900164214143No Hit
CATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG20730.12783173816014795No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT20730.12783173816014795No Hit
TCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATA20680.12752341269425274No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG20560.12678343157610428No Hit
GCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGG20510.12647510611020907No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA20490.12635177592385102No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC20180.12444015803530081No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA20000.12333018635807809No Hit
CATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTT19970.12314519107854097No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC19940.12296019579900386No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA19640.1211102430036327No Hit
ACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAA19290.11895196474236633No Hit
CTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACG19180.11827364871739689No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC19080.11765699778560651No Hit
CATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT18980.11704034685381612No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC18960.11691701666745803No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC18810.11599204026977245No Hit
CACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG18760.11568371480387726No Hit
TCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCAT18640.11494373368572879No Hit
GCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTT18560.11445041294029647No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA18380.11334044126307377No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC18330.11303211579717858No Hit
GTACATGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTG18180.11210713939949299No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA18030.1111821630018074No Hit
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG18010.11105883281544932No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA17830.10994886113822662No Hit
TTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGC17750.1094555403927943No Hit
CTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGC17680.10902388474054103No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT17470.10772891778378121No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA17380.10717393194516987No Hit
AGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCC16890.10415234237939694No Hit
CGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGC16850.1039056820066808No Hit
ATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCATTTACC16770.10341236126124849No Hit
ATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGAC16730.10316570088853233No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA16680.10285737542263713No Hit
GATATAACGCTGCATAATTGACGGACTGGCCATCAACTCGATATTTTTGT16610.10242571977038385No Hit
TCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG16590.10230238958402578No Hit
AAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA16390.101069087720445No Hit
CTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACA16380.10100742262726596No Hit
GATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCG16320.10063743206819172No Hit
CATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAG16280.10039077169547556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)