FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001323727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964260
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65120.675336527492585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56870.5897786903947069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33970.352290875904839No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG24230.25128077489473793No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG23330.24194719266587852No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT23130.23987306328168753No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA21070.21850953062452033No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT18970.196731172090515No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG18070.18739758986165556No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT17620.18273079874722584No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA17600.18252338580880675No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA17590.1824196793395972No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG17310.17951589820172983No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG16790.17412316180283327No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC16750.17370833592599508No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG15420.159915375521125No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT15320.15887831082902953No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC14810.1535892808993425No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT14780.15327816149171386No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT14750.1529670420840852No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA14390.14923360919254142No Hit
CTACTAAGATGTTTCAATTCGCAACGTGTCTCGCTAATTTGACTATGGAT14160.1468483604007218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.13813701698711967Clontech SMART CDS Primer II A (100% over 21bp)
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC12670.13139609648849895No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG12390.12849231535063158No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC12360.12818119594300292No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA11930.1237218177669923No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA11690.12123286250596312No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT11590.12019579781386762No Hit
ACTAAGATGTTTCAATTCGCAACGTGTCTCGCTAATTTGACTATGGATTC11520.11946985252940079No Hit
ATATTAAGCTGATCAATCACTTGATCAAGCAAAACAGGTATATGATCCAT11400.1182253748988862No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC11150.11563271316864746No Hit
GTCCCAGTGTGGCCGTACAGCCTCTCGGCCCGGCTAAACATCATCGTCTT11040.11449194200734243No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC10970.11376599672287557No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG10820.11221039968473234No Hit
GAATTAGGTAATGTATTTTATATTAGTCTTAGCTTAGATCAAGTACTAGA10190.10567689212453073No Hit
GGATGTAGCAATACATTCAGTAGCGAATGGGTGAGTAACACGTACCTAAC10090.10463982743243523No Hit
TACCTAACCTACCTTTAAGACTGGGATAACTATTGGAAACAATAGCTAAT9990.10360276274033973No Hit
AGCTAATACCGGATATAGTTATTTATCGCATGATGAGTAATAGAAAGGAG9970.10339534980192064No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT9960.1032916433327111No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)