FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001323728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964260
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA33020.3424387613299318No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA24700.25615497894758676No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA24370.2527326654636716No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA20200.20948706780328957No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC18570.19258291332213304No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG17180.1781677141020057No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC16840.174641694148881No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG16160.16758965424263167No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG15770.16354510194345923No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA14950.1550411714682762No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA14400.14933731566175099No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT13100.13585547466450956No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG13020.13502582291083318No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT12850.13326281293427084No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA12650.13118868355007984No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC12540.1300479123887748No Hit
CTACTAAGATGTTTCAATTCGCAACGTGTCTCGCTAATTTGACTATGGAT12510.12973679298114615No Hit
GTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAAAATTCCTTACTGCTGC12150.1260033600896024No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT11840.12278845954410637No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC11740.12175139485201088No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT11010.11418082259971377No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC10990.11397340966129467No Hit
ACTAAGATGTTTCAATTCGCAACGTGTCTCGCTAATTTGACTATGGATTC10850.11252151909236098No Hit
ATATTAAGCTGATCAATCACTTGATCAAGCAAAACAGGTATATGATCCAT10740.11138074793105594No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT10720.11117333499263685No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG10640.11034368323896045No Hit
GTCCCAGTGTGGCCGTACAGCCTCTCGGCCCGGCTAAACATCATCGTCTT10520.10909920560844585No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT10510.10899549913923631No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC10350.10733619563188353No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT10350.10733619563188353No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG10080.10453612096322569No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA10050.10422500155559704No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG10030.10401758861717794No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC9940.103084230394292No Hit
CCTTATAACAGCAGTTTACATTCCGAAGAACTTCATCCTGCACGCTGTGT9830.10194345923298695No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT9790.10152863335614874No Hit
GAATTAGGTAATGTATTTTATATTAGTCTTAGCTTAGATCAAGTACTAGA9790.10152863335614874No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG9730.10090639454089147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)